• Product name
  • Description
    Rabbit polyclonal to RBM10
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 880 and the C-terminus (residue 930) of human RBM10 (NP_005667.2)

  • Positive control
    • HeLa whole cell lysate.



Our Abpromise guarantee covers the use of ab72423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A).
  • Involvement in disease
    Defects in RBM10 are the cause of TARP syndrome (TARPS) [MIM:311900]. It is a disorder characterized by the Robin sequence (micrognathia, glossoptosis and cleft palate), talipes equinovarus and cardiac defects.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 G-patch domain.
    Contains 1 RanBP2-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. In the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. Upon globally reducing RNA polymerase II transcription, the nuclear bodies enlarge and decrease in number. They occur closely adjacent to nuclear speckles or IGCs (interchromatin granule clusters) but coincide with TIDRs.
  • Information by UniProt
  • Database links
  • Alternative names
    • DXS8237E antibody
    • G patch domain containing protein 9 antibody
    • G patch domain-containing protein 9 antibody
    • GPATC9 antibody
    • GPATCH9 antibody
    • HGNC9896 antibody
    • KIAA0122 antibody
    • MGC1132 antibody
    • MGC997 antibody
    • Rbm10 antibody
    • RBM10_HUMAN antibody
    • RNA binding motif protein 10 antibody
    • RNA binding protein 10 antibody
    • RNA binding protein S1-1 antibody
    • RNA-binding motif protein 10 antibody
    • RNA-binding protein 10 antibody
    • RNA-binding protein S1-1 antibody
    • S1-1 antibody
    • TARPS antibody
    • ZRANB5 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling RBM10 with ab72423 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-RBM10 antibody (ab72423) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 104 kDa
    Observed band size: 120 kDa (why is the actual band size different from the predicted?)

    Exposure time: 3 minutes
  • Detection of RBM10 by Western Blot of Immunprecipitate.
    ab72423 at 1µg/ml staining RBM10 in HeLa whole cell lysate immunoprecipitated using ab72423 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.


This product has been referenced in:
  • Rodor J  et al. The RNA-binding landscape of RBM10 and its role in alternative splicing regulation in models of mouse early development. RNA Biol 14:45-57 (2017). WB, CLIP ; Mouse . Read more (PubMed: 27763814) »

See 1 Publication for this product

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