Key features and details
- Rabbit polyclonal to RBM10/S1-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RBM10/S1-1 antibody
See all RBM10/S1-1 primary antibodies
DescriptionRabbit polyclonal to RBM10/S1-1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- Daudi cell lysate. Human heart or lung sections.
Protein previously labeled as RBM10.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab26046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1.4 µg/ml. Predicted molecular weight: 104 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionMay be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A).
Involvement in diseaseDefects in RBM10 are the cause of TARP syndrome (TARPS) [MIM:311900]. It is a disorder characterized by the Robin sequence (micrognathia, glossoptosis and cleft palate), talipes equinovarus and cardiac defects.
Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 G-patch domain.
Contains 1 RanBP2-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. In the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. Upon globally reducing RNA polymerase II transcription, the nuclear bodies enlarge and decrease in number. They occur closely adjacent to nuclear speckles or IGCs (interchromatin granule clusters) but coincide with TIDRs.
- Information by UniProt
- DXS8237E antibody
- G patch domain containing protein 9 antibody
- G patch domain-containing protein 9 antibody
Anti-RBM10/S1-1 antibody (ab26046) at 0.5 µg/ml + Daudi cell lysate
HRP conjugated anti-Rabbit IgG diluted 1: 50,000 - 100,000
Predicted band size: 104 kDa
Staining of human heart tissue with ab26046 at a 4.0-8.0ug/ml dilution. Positive labelled myocardial cells are indicated with arrows.
Staining of human lung tissue with ab26046 at a 4.0-8.0ug/ml dilution. Positive labelled epithelial cells of bronchiole are indicated with arrows.
ab26046 has been referenced in 3 publications.
- Guan G et al. Expression of RNA-binding motif 10 is associated with advanced tumor stage and malignant behaviors of lung adenocarcinoma cancer cells. Tumour Biol 39:1010428317691740 (2017). PubMed: 28347232
- Ji Y et al. Increased cell apoptosis in human lung adenocarcinoma andin vivotumor growth inhibition byRBM10, a tumor suppressor gene. Oncol Lett 14:4663-4669 (2017). PubMed: 29085465
- Wang Y et al. Integrative analysis revealed the molecular mechanism underlying RBM10-mediated splicing regulation. EMBO Mol Med 5:1431-42 (2013). WB . PubMed: 24000153