Key features and details
- Rabbit polyclonal to RBM10/S1-1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RBM10/S1-1 antibody
See all RBM10/S1-1 primary antibodies
DescriptionRabbit polyclonal to RBM10/S1-1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to Human RBM10/S1-1 (C terminal).
- HeLa whole cell lysate.
Protein previously labeled as RBM10.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72423 was affinity purified using an epitope specific to RBM10/S1-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A).
Involvement in diseaseDefects in RBM10 are the cause of TARP syndrome (TARPS) [MIM:311900]. It is a disorder characterized by the Robin sequence (micrognathia, glossoptosis and cleft palate), talipes equinovarus and cardiac defects.
Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 G-patch domain.
Contains 1 RanBP2-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. In the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. Upon globally reducing RNA polymerase II transcription, the nuclear bodies enlarge and decrease in number. They occur closely adjacent to nuclear speckles or IGCs (interchromatin granule clusters) but coincide with TIDRs.
- Information by UniProt
- DXS8237E antibody
- G patch domain containing protein 9 antibody
- G patch domain-containing protein 9 antibody
All lanes : Anti-RBM10/S1-1 antibody (ab72423) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling RBM10/S1-1 with ab72423 at 1/200 (1µg/ml). Detection: DAB.
Detection of RBM10/S1-1 by Western Blot of Immunprecipitate.
ab72423 at 1µg/ml staining RBM10/S1-1 in HeLa whole cell lysate immunoprecipitated using ab72423 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab72423 has been referenced in 3 publications.
- Sun X et al. Functional role of RBM10 in lung adenocarcinoma proliferation. Int J Oncol 54:467-478 (2019). PubMed: 30483773
- Rodor J et al. The RNA-binding landscape of RBM10 and its role in alternative splicing regulation in models of mouse early development. RNA Biol 14:45-57 (2017). WB, CLIP ; Mouse . PubMed: 27763814
- Treiber T et al. A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis. Mol Cell 66:270-284.e13 (2017). PubMed: 28431233