Key features and details
- Rabbit polyclonal to RBM10/S1-1 - N-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RBM10/S1-1 antibody - N-terminal
See all RBM10/S1-1 primary antibodies
DescriptionRabbit polyclonal to RBM10/S1-1 - N-terminal
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: A549 cell lysate. IHC-P: Human cerebellum, prostate, small intestine and skeletal muscle tissues. ICC/IF: A431 cells.
Protein previously labeled as RBM10.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 94,103,104 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A).
Involvement in diseaseDefects in RBM10 are the cause of TARP syndrome (TARPS) [MIM:311900]. It is a disorder characterized by the Robin sequence (micrognathia, glossoptosis and cleft palate), talipes equinovarus and cardiac defects.
Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 G-patch domain.
Contains 1 RanBP2-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. In the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. Upon globally reducing RNA polymerase II transcription, the nuclear bodies enlarge and decrease in number. They occur closely adjacent to nuclear speckles or IGCs (interchromatin granule clusters) but coincide with TIDRs.
- Information by UniProt
- DXS8237E antibody
- G patch domain containing protein 9 antibody
- G patch domain-containing protein 9 antibody
Anti-RBM10/S1-1 antibody - N-terminal (ab224149) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 94,103,104 kDa
Paraffin-embedded human cerebellum tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.
Shows moderate to strong nuclear positivity in glandular cells.
Paraffin-embedded human skeletal muscle tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RBM10/S1-1 (green) using ab224149 at 4 µg/ml in ICC/IF.
ab224149 has not yet been referenced specifically in any publications.