Overview

  • Product name

    Anti-RBM10/S1-1 antibody - N-terminal
    See all RBM10/S1-1 primary antibodies
  • Description

    Rabbit polyclonal to RBM10/S1-1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human RBM10/S1-1 aa 1-81 (N terminal).
    Sequence:

    MEYERRGGRGDRTGRYGATDRSQDDGGENRSRDHDYRDMDYRSYPREYGS QEGKHDYDDSSEEQSAEDSYEASPGSETQRR


    Database link: P98175

  • Positive control

    • WB: A549 cell lysate. IHC-P: Human cerebellum, prostate, small intestine and skeletal muscle tissues. ICC/IF: A431 cells.
  • General notes

    Protein previously labeled as RBM10.

Properties

Applications

Our Abpromise guarantee covers the use of ab224149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB 1/100 - 1/250. Predicted molecular weight: 94,103,104 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A).
  • Involvement in disease

    Defects in RBM10 are the cause of TARP syndrome (TARPS) [MIM:311900]. It is a disorder characterized by the Robin sequence (micrognathia, glossoptosis and cleft palate), talipes equinovarus and cardiac defects.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 G-patch domain.
    Contains 1 RanBP2-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. In the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. Upon globally reducing RNA polymerase II transcription, the nuclear bodies enlarge and decrease in number. They occur closely adjacent to nuclear speckles or IGCs (interchromatin granule clusters) but coincide with TIDRs.
  • Information by UniProt
  • Database links

  • Alternative names

    • DXS8237E antibody
    • G patch domain containing protein 9 antibody
    • G patch domain-containing protein 9 antibody
    • GPATC9 antibody
    • GPATCH9 antibody
    • HGNC9896 antibody
    • KIAA0122 antibody
    • MGC1132 antibody
    • MGC997 antibody
    • Rbm10 antibody
    • RBM10_HUMAN antibody
    • RNA binding motif protein 10 antibody
    • RNA binding protein 10 antibody
    • RNA binding protein S1-1 antibody
    • RNA-binding motif protein 10 antibody
    • RNA-binding protein 10 antibody
    • RNA-binding protein S1-1 antibody
    • S1-1 antibody
    • TARPS antibody
    • ZRANB5 antibody
    see all

Images

  • Anti-RBM10/S1-1 antibody - N-terminal (ab224149) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 94,103,104 kDa

  • Paraffin-embedded human cerebellum tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human prostate tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human small intestine tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human skeletal muscle tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

  • PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RBM10/S1-1 (green) using ab224149 at 4 µg/ml in ICC/IF.

References

ab224149 has not yet been referenced specifically in any publications.

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