Key features and details
- Rabbit polyclonal to RBM14
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RBM14 antibody
See all RBM14 primary antibodies
DescriptionRabbit polyclonal to RBM14
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Immunogen was a synthetic peptide, which represented a portion of human Coactivator Activator encoded within exon 2 (LocusLink ID 10432).
- Nuclear extract from HeLa cells.
General notesCentrifuge tube to remove product from lid.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified using the peptide immobilized on solid support.
Our Abpromise guarantee covers the use of ab12325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 80 kDa. Prepare working dilutions immediately before use.|
RelevanceRBM14 is a RNA-binding protein. These proteins contribute to gene expression by regulating the form, abundance and stability of both coding and non-coding RNAs. In vertebrates, RNA binding proteins can also influence many processes involved in RNA processing, for example, activity-dependent transcript localization and localized protein synthesis amongst others.
- CoAA antibody
- Coactivator Activator antibody
- RBM 14 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling RBM14 with ab12325 at 1/1000 (1µg/ml). Detection: DAB.
Detection of Human CoAA by Western Blot.
Samples: Nuclear extract (NE) from HeLa cells.
Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Detection of Human CoAA by Western Blot. Samples: Nuclear extract (NE) from HeLa cells. Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
ICC/IF image of ab12325 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12325, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab12325 has been referenced in 2 publications.
- Shiratsuchi G et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . PubMed: 25385835
- Li X et al. Co-activator activator (CoAA) prevents the transcriptional activity of Runt domain transcription factors. J Cell Biochem 108:378-87 (2009). PubMed: 19585539