Overview

  • Product name

  • Description

    Rabbit polyclonal to Rbm15/OTT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Rbm15/OTT aa 264-378.
    Sequence:

    DTYPPSASVVGASVGGHRHPPGGGGGQRSLSPGGAALGYRDYRLQQLALG RLPPPPPPPLPRDLERERDYPFYERVRPAYSLEPRVGAGAGAAPFREVDE ISPEDDQRANRTLFL


    Database link: Q96T37

  • Positive control

    • IHC-P: Human colon tissue. WB: K562, NIH/3T3 and NBT-II cell lysate. ICC/IF: U-2 OS cells.
  • General notes

     This product was previously labelled as Rbm15

     

Properties

Applications

Our Abpromise guarantee covers the use of ab244374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB Use a concentration of 0.4 µg/ml.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be implicated in HOX gene regulation.
  • Involvement in disease

    A chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
  • Sequence similarities

    Belongs to the RRM Spen family.
    Contains 3 RRM (RNA recognition motif) domains.
    Contains 1 SPOC domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • One twenty two protein antibody
    • One-twenty two protein 1 antibody
    • OTT antibody
    • OTT1 antibody
    • Putative RNA-binding protein 15 antibody
    • RBM15 antibody
    • RBM15_HUMAN antibody
    • RNA binding motif protein 15 antibody
    • RNA-binding motif protein 15 antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Rbm15/OTT (green) using ab244374 at 4 µg/ml in ICC/IF.

  • Paraffin-embedded human colon tissue stained for Rbm15/OTT using ab244374 at 1/20 dilution in immunohistochemical analysis.

  • Anti-Rbm15/OTT antibody (ab244374) at 0.4 µg/ml + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
  • All lanes : Anti-Rbm15/OTT antibody (ab244374) at 0.4 µg/ml

    Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
    Lane 2 : NBT-II (Rat cell line) cell lysate

References

This product has been referenced in:

  • Huang B  et al. Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model. Front Endocrinol (Lausanne) 10:415 (2019). Read more (PubMed: 31316467) »
See 1 Publication for this product

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