Key features and details
- Rabbit polyclonal to Rbm15/OTT
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Rbm15/OTT antibody
See all Rbm15/OTT primary antibodies
DescriptionRabbit polyclonal to Rbm15/OTT
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- IHC-P: Human colon tissue. WB: K562, NIH/3T3 and NBT-II cell lysate. ICC/IF: U-2 OS cells.
This product was previously labelled as Rbm15
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be implicated in HOX gene regulation.
Involvement in diseaseA chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
Sequence similaritiesBelongs to the RRM Spen family.
Contains 3 RRM (RNA recognition motif) domains.
Contains 1 SPOC domain.
- Information by UniProt
- One twenty two protein antibody
- One-twenty two protein 1 antibody
- OTT antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Rbm15/OTT (green) using ab244374 at 4 µg/ml in ICC/IF.
Formalin-fixed, paraffin-embedded human lymph node tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human testis tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human placenta tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
Anti-Rbm15/OTT antibody (ab244374) at 0.4 µg/ml + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
All lanes : Anti-Rbm15/OTT antibody (ab244374) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244374 has been referenced in 1 publication.
- Huang B et al. Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model. Front Endocrinol (Lausanne) 10:415 (2019). PubMed: 31316467