Key features and details
- Rabbit polyclonal to Rbm15/OTT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Rbm15/OTT antibody
See all Rbm15/OTT primary antibodies
DescriptionRabbit polyclonal to Rbm15/OTT
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
Synthetic peptide corresponding to Human Rbm15/OTT.
This product was previously labelled as Rbm15
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay be implicated in HOX gene regulation.
Involvement in diseaseA chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
Sequence similaritiesBelongs to the RRM Spen family.
Contains 3 RRM (RNA recognition motif) domains.
Contains 1 SPOC domain.
- Information by UniProt
- One twenty two protein antibody
- One-twenty two protein 1 antibody
- OTT antibody
All lanes : Anti-Rbm15/OTT antibody (ab70549) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 3 minutes
Detection of Human Rbm15/OTT by Western Blot of Immunprecipitate. ab70549 at 1µg/ml staining Rbm15/OTT in HeLa whole cell lysate immunoprecipitated by ab70549 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
ab70549 has been referenced in 4 publications.
- Wang CY et al. PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments. Nat Commun 10:2950 (2019). PubMed: 31270318
- Jin S et al. PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+cells. Exp Ther Med 15:2563-2568 (2018). PubMed: 29456659
- Knuckles P et al. Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d. Genes Dev 32:415-429 (2018). PubMed: 29535189
- Horiuchi K et al. Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J Biol Chem 288:33292-302 (2013). WB, ICC/IF ; Human . PubMed: 24100041