Product nameAnti-Rbm15/OTT antibody
See all Rbm15/OTT primary antibodies
DescriptionRabbit polyclonal to Rbm15/OTT
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
Synthetic peptide corresponding to Human Rbm15/OTT.
- HeLa and 293T whole cell lysates.
This product was previously labelled as Rbm15
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 24100041|
|WB||1/2000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay be implicated in HOX gene regulation.
Involvement in diseaseA chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
Sequence similaritiesBelongs to the RRM Spen family.
Contains 3 RRM (RNA recognition motif) domains.
Contains 1 SPOC domain.
- Information by UniProt
- One twenty two protein antibody
- One-twenty two protein 1 antibody
- OTT antibody
All lanes : Anti-Rbm15/OTT antibody (ab70549) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 3 minutes
Immunocytochemistry/Immunofluorescence analysis of HUVEC cells labelling Rbm15/OTT (red) with ab70549 at 1:100. Cells were fixed with 4% paraformaldehyde in PBS for 5 minutes, permeabilized with 0.5% Triton X-100 in PBS for 5 minutes on ice, blocked with PBS containing 10% BlockAce and incubated with the primary antibodies. An Alexa Fluor 594-conjugated anti-rabbit IgG was used as the secondary antibody. SC35 - green. Rbm15/OTT and SC35 are partially co-localized in nuclear speckles and are also present in the the nucleoplasm.
Detection of Human Rbm15/OTT by Western Blot of Immunprecipitate. ab70549 at 1µg/ml staining Rbm15/OTT in HeLa whole cell lysate immunoprecipitated by ab70549 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
This product has been referenced in:
- Jin S et al. PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+cells. Exp Ther Med 15:2563-2568 (2018). Read more (PubMed: 29456659) »
- Knuckles P et al. Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d. Genes Dev 32:415-429 (2018). Read more (PubMed: 29535189) »