Overview

  • Product name

  • Description

    Rabbit polyclonal to Rbm15/OTT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human Rbm15/OTT.

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

     This product was previously labelled as Rbm15

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24100041
WB 1/2000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    May be implicated in HOX gene regulation.
  • Involvement in disease

    A chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
  • Sequence similarities

    Belongs to the RRM Spen family.
    Contains 3 RRM (RNA recognition motif) domains.
    Contains 1 SPOC domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • One twenty two protein antibody
    • One-twenty two protein 1 antibody
    • OTT antibody
    • OTT1 antibody
    • Putative RNA-binding protein 15 antibody
    • RBM15 antibody
    • RBM15_HUMAN antibody
    • RNA binding motif protein 15 antibody
    • RNA-binding motif protein 15 antibody
    see all

Images

  • All lanes : Anti-Rbm15/OTT antibody (ab70549) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 107 kDa
    Observed band size: 107 kDa


    Exposure time: 3 minutes
  • Immunocytochemistry/Immunofluorescence analysis of HUVEC cells labelling Rbm15/OTT (red) with ab70549 at 1:100. Cells were fixed with 4% paraformaldehyde in PBS for 5 minutes, permeabilized with 0.5% Triton X-100 in PBS for 5 minutes on ice, blocked with PBS containing 10% BlockAce and incubated with the primary antibodies. An Alexa Fluor 594-conjugated anti-rabbit IgG was used as the secondary antibody. SC35 - green. Rbm15/OTT and SC35 are partially co-localized in nuclear speckles and are also present in the the nucleoplasm.

  • Detection of Human Rbm15/OTT by Western Blot of Immunprecipitate. ab70549 at 1µg/ml staining Rbm15/OTT in HeLa whole cell lysate immunoprecipitated by ab70549 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

References

This product has been referenced in:

  • Jin S  et al. PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+cells. Exp Ther Med 15:2563-2568 (2018). Read more (PubMed: 29456659) »
  • Knuckles P  et al. Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d. Genes Dev 32:415-429 (2018). Read more (PubMed: 29535189) »
See all 3 Publications for this product

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