Product nameAnti-RBM19 antibody
DescriptionRabbit polyclonal to RBM19
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human stomach tissue, A431 cells; RT4 and U251 MG cell lysates; Human tonsil lysate.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionPlays a role in embryo pre-implantation development.
Tissue specificityExpressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia (at protein level).
Sequence similaritiesBelongs to the RRM MRD1 family.
Contains 6 RRM (RNA recognition motif) domains.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Chromosome. In discrete foci distributed throughout the cytoplasm and nucleoplasm during the 4 to 8 cell stages and the morula stage, but not in the periphery of the nucleolar precursor body (NPB). During blastocyst development, becomes increasingly localized to the nucleolus and less to the cytoplasm. At the late blastocyst stage, localized predominantly in the nucleolus. Localized in the nucleolus during interphase and to the perichromosomal sheath during mitosis. Does not colocalize in the cytoplasm with GW182 in P-bodies. May translocate to the nucleolus upon early embryonic development (By similarity). Colocalizes with NPM1 during interphase. By late prophase, metaphase, anaphase and telophase, associates with the chromosome periphery. By telophase localizes to NPB.
- Information by UniProt
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All lanes : Anti-RBM19 antibody (ab122515) at 1/100 dilution
Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
ab122515 at 1/1000 dilution staining RBM19 in Paraffin-embedded Human Stomach tissue by Immunohistochemistry.
Note: moderate cytoplasmic and nuclear positivity in glandular cells.
ab122515 at 4 µg/ml staining RBM19 in A431 cells by Immunofluorescence (green).
Note: positivity in nucleus but not nucleoli & cytoplasm.
All lanes : Anti-RBM19 antibody (ab122515) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MGlysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique
ab122515 has been referenced in 1 publication.
- Kinterova V et al. Inhibition of SCF complexes during bovine oocyte maturation and preimplantation development leads to delayed development of embryos. Biol Reprod N/A:N/A (2018). PubMed: 30535233