Overview

  • Product name

  • Description

    Rabbit polyclonal to RBM20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RBM20 aa 402-448 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    FHGVAPLHLPHICSICDKKVFDLKDWELHVKGKLHAQKCLVFSENAG


    Database link: Q5T481

  • Positive control

    • Rat heart tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat heart labeling RBM20 with ab214366 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

References

ab214366 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
60 µg
Specification
skeletal muscle
Blocking step
blocking solution from Fisher scientific cat# 37530 as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 02 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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