Key features and details
- Rabbit polyclonal to RBM20
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RBM20 antibody
See all RBM20 primary antibodies
DescriptionRabbit polyclonal to RBM20
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment (His-tag) corresponding to Human RBM20 aa 1089-1227. Expressed in E.coli. N-terminal tag.
PIETDLQNQACQEVLTPENSRYVEMKSLEVRSPEYTEVELKQPLSLPSWE PEDVFSELSIPLGVEFVVPRTGFYCKLCGLFYTSEETAKMSHCRSAVHYR NLQKYLSQLAEEGLKETEGADSPRPEDSGIVPRFERKKL
Database link: Q5T481
- IHC-P: Human breast cancer tissue. WB: Pig skeletal muscle lysate; Recombinant human RBM20 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesab233147 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 134 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
Tissue specificityExpressed in the heart.
Involvement in diseaseDefects in RBM20 are the cause of cardiomyopathy dilated type 1DD (CMD1DD) [MIM:613172]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- Probable RNA-binding protein 20 antibody
- Rbm20 antibody
- RBM20_HUMAN antibody
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for RBM20 using ab233147 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-RBM20 antibody (ab233147) at 1.5 µg/ml + Pig skeletal muscle lysate
HRP-linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 134 kDa
Anti-RBM20 antibody (ab233147) at 2 µg/ml + Recombinant human RBM20 protein
Predicted band size: 134 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233147 has not yet been referenced specifically in any publications.