Description

  • Product name

    RBM20 peptide
  • Animal free

    No
  • Nature

    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab126284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Recombinant Human ITLN1 protein (denatured) (ab109850)

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Reconstitution
    Resuspend in water or in neutral buffer

General Info

  • Alternative names

    • Probable RNA-binding protein 20
    • Rbm20
    • RBM20_HUMAN
    • RNA binding protein 20
    • RNA-binding motif protein 20
    see all
  • Tissue specificity

    Expressed in the heart.
  • Involvement in disease

    Defects in RBM20 are the cause of cardiomyopathy dilated type 1DD (CMD1DD) [MIM:613172]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Information by UniProt

References

ab126284 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your email.

I can confirm that this lyophilised peptide should be reconstituted with sterile water. If 100ul sterile water are used the concentration will be 1mg/ml.

We recommend then to aliquot and store it at -20C.

I hope this information ishelpful and wish you good luck with your experiments.

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Question
Answer

Thank you for your inquiry.

This is the protocol we would like to suggest for the blocking experiment:

1. Determine the optimal concentration of antibody that consistently gives a positive result in your particular protocol.

2. Dilute the necessary amount of antibody in blocking buffer (3% BSA in PBST)to the final volume needed for the two experiments. Divide this equally into two tubes.

3. In the first tube, labeled "Blocked", add4 timesmoreblocking peptide than antibody(example: 1ug of antibody used, then add 4ug of total peptide)
In the second tube, labeled "Control", add an equivalent amount of buffer.

4. Incubate both tubes, with agitation,overnight at 4C (or at room temperature for 30 minutes).

5. Perform the staining protocol on the two identical samples, using the blocked antibody for one and the control for the other.

6. Observe the staining. The staining that disappears when using the blocked antibody is specific to the antibody.

I hope this information will be helpful. More informationcan be found onour protocolpages:

https://www.abcam.com/index.html?pageconfig=popular_protocols

We are lookingforwardto your order.

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Answer

Thank you for your inquiry.


I can confirm that this antibody was tested with blocking peptide and the band at 25kDa did not vanish and is therefore considered unspecific. I attached an image of the blot for your information. Lane B is with the blocking peptide.


We included the blocking peptide in our catalogue for you and it isnow available for purchase with the product number ab126284.

https://www.abcam.com/index.html?datasheet=126284 (or use the following: https://www.abcam.com/index.html?datasheet=126284).


I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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