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I’m going to order the peptide, can you tell me in detail how was used? Thanks
Asked on Feb 13 2012
Thank you for your inquiry.
This is the protocol we would like to suggest for the blocking experiment:
1. Determine the optimal concentration of antibody that consistently gives a positive result in your particular protocol.
2. Dilute the necessary amount of antibody in blocking buffer (3% BSA in PBST)to the final volume needed for the two experiments. Divide this equally into two tubes.
3. In the first tube, labeled "Blocked", add4 timesmoreblocking peptide than antibody(example: 1ug of antibody used, then add 4ug of total peptide)
In the second tube, labeled "Control", add an equivalent amount of buffer.
4. Incubate both tubes, with agitation,overnight at 4C (or at room temperature for 30 minutes).
5. Perform the staining protocol on the two identical samples, using the blocked antibody for one and the control for the other.
6. Observe the staining. The staining that disappears when using the blocked antibody is specific to the antibody.
I hope this information will be helpful. More informationcan be found onour protocolpages:
We are lookingforwardto your order.
Answered on Feb 13 2012