Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses.
Belongs to the SLT11 family. Contains 1 C3H1-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.
The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding.
Nucleus. Cytoplasm. Mainly located in the nucleus. Translocated from the nucleus to the cytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol.
Functional spliceosome associated protein 47 antibody
Pre mRNA splicing factor RBM22 antibody
Pre-mRNA-splicing factor RBM22 antibody
RBM 22 antibody
RNA binding motif protein 22 antibody
RNA-binding motif protein 22 antibody
Zinc finger CCCH domain containing protein 16 antibody
Zinc finger CCCH domain-containing protein 16 antibody
Western blot - Anti-RBM22 antibody (ab157105)
All lanes : Anti-RBM22 antibody (ab157105) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Detection of RBM22 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157105 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157105 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.