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    rbm22-antibody-ab157105.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
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Anti-RBM22 antibody (ab157105)

  • Datasheet
  • SDS
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Western blot - Anti-RBM22 antibody (ab157105)
  • Immunoprecipitation - Anti-RBM22 antibody (ab157105)

Key features and details

  • Rabbit polyclonal to RBM22
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RBM22 antibody
  • Description

    Rabbit polyclonal to RBM22
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human RBM22 (NP_060517.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab157105 was affinity purified using an epitope specific to RBM22 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH/3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Rat
Rabbit
Horse
Chicken
Guinea pig
Cow
Dog
Turkey
Pig
Xenopus laevis
Chimpanzee
Zebrafish
Rhesus monkey
Gorilla
Orangutan
Xenopus tropicalis
Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses.
  • Sequence similarities

    Belongs to the SLT11 family.
    Contains 1 C3H1-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain

    The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding.
  • Cellular localization

    Nucleus. Cytoplasm. Mainly located in the nucleus. Translocated from the nucleus to the cytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol.
  • Target information above from: UniProt accession Q9NW64 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 416270 Chicken
    • Entrez Gene: 471698 Chimpanzee
    • Entrez Gene: 512330 Cow
    • Entrez Gene: 489184 Dog
    • Entrez Gene: 55696 Human
    • Entrez Gene: 66810 Mouse
    • Entrez Gene: 100172542 Orangutan
    • Entrez Gene: 100345063 Rabbit
    • Entrez Gene: 307410 Rat
    • Entrez Gene: 712713 Rhesus monkey
    • Entrez Gene: 380469 Xenopus laevis
    • Entrez Gene: 394713 Xenopus tropicalis
    • Entrez Gene: 100536641 Zebrafish
    • Entrez Gene: 406495 Zebrafish
    • Omim: 612430 Human
    • SwissProt: Q5ZM16 Chicken
    • SwissProt: Q3B7L8 Cow
    • SwissProt: Q9NW64 Human
    • SwissProt: Q8BHS3 Mouse
    • SwissProt: Q5RAY5 Orangutan
    • SwissProt: Q4V7D7 Rat
    • SwissProt: Q7ZXB5 Xenopus laevis
    • SwissProt: Q6P616 Xenopus tropicalis
    • SwissProt: Q6NZZ9 Zebrafish
    • Unigene: 713564 Human
    • Unigene: 275106 Mouse
    • Unigene: 105130 Rat
    • Unigene: 4959 Xenopus laevis
    • Unigene: 32898 Zebrafish
    see all
  • Alternative names

    • Cwc2 antibody
    • FLJ10290 antibody
    • fSAP47 antibody
    • Functional spliceosome associated protein 47 antibody
    • Pre mRNA splicing factor RBM22 antibody
    • Pre-mRNA-splicing factor RBM22 antibody
    • RBM 22 antibody
    • rbm22 antibody
    • RBM22_HUMAN antibody
    • RNA binding motif protein 22 antibody
    • RNA-binding motif protein 22 antibody
    • ZC3H16 antibody
    • Zinc finger CCCH domain containing protein 16 antibody
    • Zinc finger CCCH domain-containing protein 16 antibody
    see all

Images

  • Western blot - Anti-RBM22 antibody (ab157105)
    Western blot - Anti-RBM22 antibody (ab157105)
    All lanes : Anti-RBM22 antibody (ab157105) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 47 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-RBM22 antibody (ab157105)
    Immunoprecipitation - Anti-RBM22 antibody (ab157105)
    Detection of RBM22 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157105 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157105 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab157105? Please let us know so that we can cite the reference in this datasheet.

ab157105 has not yet been referenced specifically in any publications.

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