Anti-RBM22 antibody (ab157105)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RBM22 antibody -
Description
Rabbit polyclonal to RBM22 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human RBM22 (NP_060517.1).
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Positive control
- 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab157105 was affinity purified using an epitope specific to RBM22 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 47 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses. -
Sequence similarities
Belongs to the SLT11 family.
Contains 1 C3H1-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding. -
Cellular localization
Nucleus. Cytoplasm. Mainly located in the nucleus. Translocated from the nucleus to the cytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol. - Information by UniProt
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Database links
- Entrez Gene: 416270 Chicken
- Entrez Gene: 471698 Chimpanzee
- Entrez Gene: 512330 Cow
- Entrez Gene: 489184 Dog
- Entrez Gene: 55696 Human
- Entrez Gene: 66810 Mouse
- Entrez Gene: 100172542 Orangutan
- Entrez Gene: 100345063 Rabbit
see all -
Alternative names
- Cwc2 antibody
- FLJ10290 antibody
- fSAP47 antibody
see all
Images
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Western blot - Anti-RBM22 antibody (ab157105)All lanes : Anti-RBM22 antibody (ab157105) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-RBM22 antibody (ab157105)Detection of RBM22 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157105 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157105 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
Datasheets and documents
References
ab157105 has not yet been referenced specifically in any publications.
