Anti-RBM22 antibody (ab157105)

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Western blot - Anti-RBM22 antibody (ab157105)
  • Immunoprecipitation - Anti-RBM22 antibody (ab157105)

Key features and details

  • Rabbit polyclonal to RBM22
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RBM22 antibody

  • Description

    Rabbit polyclonal to RBM22

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human RBM22 (NP_060517.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab157105 was affinity purified using an epitope specific to RBM22 immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Rat
Rabbit
Horse
Chicken
Guinea pig
Cow
Dog
Turkey
Pig
Xenopus laevis
Chimpanzee
Zebrafish
Rhesus monkey
Gorilla
Orangutan
Xenopus tropicalis
Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

Images

  • Western blot - Anti-RBM22 antibody (ab157105)
    Western blot - Anti-RBM22 antibody (ab157105)
    All lanes : Anti-RBM22 antibody (ab157105) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 47 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-RBM22 antibody (ab157105)
    Immunoprecipitation - Anti-RBM22 antibody (ab157105)
    Detection of RBM22 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157105 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157105 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.

References (0)

Publishing research using ab157105? Please let us know so that we can cite the reference in this datasheet.

ab157105 has not yet been referenced specifically in any publications.

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