Overview

  • Product name

    Anti-RBM24 antibody
  • Description

    Rabbit polyclonal to RBM24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 144-191 (TAGGYGYAVQ QPITAAAPGT AAAAAAAAAA AAAFGQYQPQ QLQTDRMQ) of Human RBM24 (NP_694565).

  • Positive control

    • COLO205 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. (Isoform 2) Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Western blot abalysis of Human fetal liver tissue lysate labeling RBM24 with ab94567 at 1.0µg/ml.
  • Anti-RBM24 antibody (ab94567) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 19 kDa



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling RBM24 with ab94567 at 5µg/ml.

References

This product has been referenced in:

  • Yao Y  et al. RBM24 stabilizes hepatitis B virus pregenomic RNA but inhibits core protein translation by targeting the terminal redundancy sequence. Emerg Microbes Infect 7:86 (2018). Read more (PubMed: 29760415) »
  • Bruun GH  et al. Blocking of an intronic splicing silencer completely rescues IKBKAP exon 20 splicing in familial dysautonomia patient cells. Nucleic Acids Res 46:7938-7952 (2018). Read more (PubMed: 29762696) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12, induced the differentiation by medium with)
Gel Running Conditions
Reduced Denaturing (gradient 10-20% e-Pagel (Atto))
Loading amount
30 µg
Specification
C2C12, induced the differentiation by medium with
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 01 2015

Answer

ab94567 is only tested in western blot by whole cell lysate. At the moment we are unaware of any publications. Localization information is based on http://www.uniprot.org/uniprot/Q9BX46.

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