Overview

  • Product name
    Anti-RBM24 antibody - N-terminal
    See all RBM24 primary antibodies
  • Description
    Rabbit polyclonal to RBM24 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • Mouse heart tissue lysate; Human prostate carcinoma tissu; 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab174919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 24 kDa.

Target

  • Function
    Plays a role in myogenic differentiation by regulating MYOG levels. Binds to the 3'-UTR of MYOG mRNA and regulates its stability.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ259A10.1 antibody
    • FLJ26355 antibody
    • FLJ30829 antibody
    • FLJ37697 antibody
    • RBM 24 antibody
    • rbm24 antibody
    • RBM24_HUMAN antibody
    • RNA binding motif protein 24 antibody
    • RNA binding protein 24 antibody
    • RNA binding region (RNP1 RRM) containing 6 antibody
    • RNA binding region containing protein 6 antibody
    • RNA-binding motif protein 24 antibody
    • RNA-binding protein 24 antibody
    • RNA-binding region-containing protein 6 antibody
    • RNPC6 antibody
    see all

Images

  • Anti-RBM24 antibody - N-terminal (ab174919) at 1/100 dilution + Mouse heart tissue lysate at 15 µg

    Predicted band size: 24 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate carcinoma tissue labeling RBM24 using ab174919 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram) using ab174919 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab174919 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Zebrafish whole embryo)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
47500 µg
Specification
Zebrafish whole embryo
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Ms. Lindy Brastrom

Verified customer

Submitted Jan 04 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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