Overview

  • Product name

    Anti-RBM25 antibody
  • Description

    Rabbit polyclonal to RBM25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus
  • Immunogen

    Synthetic peptide from a region between residue 475 and 525 of human RBM25

  • Positive control

    • Whole cell lysate from Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 71, 117 kDa (predicted molecular weight: 94 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA.
  • Sequence similarities

    Contains 1 PWI domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Nucleus speckle. Cytoplasm. Colocalizes predominantly, with SFRS2 and LUC7L3 splicing factors, in nuclear speckles. Cytoplasmic localization is faint.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arg/Glu/Asp rich protein, 120 kDa antibody
    • Arg/Glu/Asp-rich protein of 120 kDa antibody
    • fSAP94 antibody
    • functional spliceosome-associated protein 94 antibody
    • NET52 antibody
    • Protein S164 antibody
    • Rbm25 antibody
    • RBM25_HUMAN antibody
    • RED120 antibody
    • RNA binding motif protein 25 antibody
    • RNA binding region (RNP1, RRM) containing 7 antibody
    • RNA-binding motif protein 25 antibody
    • RNA-binding protein 25 antibody
    • RNA-binding region-containing protein 7 antibody
    • RNPC7 antibody
    • S164 antibody
    • Snu71 antibody
    • U1 small nuclear ribonucleoprotein 1SNRP homolog antibody
    see all

Images

  • All lanes : Anti-RBM25 antibody (ab72237) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg

    Predicted band size: 94 kDa
    Observed band size: 117 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.



    Various other bands are detected.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RBM25 with ab72237 at 1/200 (1 µg/ml). Detection: DAB.
  • Immunoprecipitation/ Western Blot of RBM25.
    Lane 1: ab72237 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72237 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 3 seconds.

References

This product has been referenced in:

  • Velazquez R  et al. Maternal choline supplementation ameliorates Alzheimer's disease pathology by reducing brain homocysteine levels across multiple generations. Mol Psychiatry N/A:N/A (2019). Read more (PubMed: 30622336) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (hESC)
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Loading amount
20 µg
Specification
hESC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Miguel Fidalgo

Verified customer

Submitted Nov 13 2015

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