Overview

  • Product name
  • Description
    Rabbit polyclonal to RBM26
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 200 and 250 of human RBM26 (NP_071401.3)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab74197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    RBM26 (RNA binding motif protein 26) bears two RNA recognition motif (RRM) domains and a C3H1-type zinc finger. The function remains unknown.
  • Database links
  • Alternative names
    • 1700009P03Rik antibody
    • acidic rich RS domain containing 2 antibody
    • AI447318 antibody
    • ARRS2 antibody
    • C13orf10 antibody
    • C230097K14Rik antibody
    • C86225 antibody
    • CTCL tumor antigen se70 2 antibody
    • cutaneous T-cell lymphoma tumor antigen se70-2 antibody
    • FLJ20957 antibody
    • MGC133295 antibody
    • MGC133296 antibody
    • PRO1777 antibody
    • protein expressed in male leptotene and zygotene spermatocytes 393 antibody
    • RNA binding motif protein 26 antibody
    • RNA binding protein 26 antibody
    • RP11 255E21.1 antibody
    • SE70 2 antibody
    • ZC3H17 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling RBM26 with ab74197 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-RBM26 antibody (ab74197) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 114 kDa
    Observed band size: 125 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 seconds
  • Detection of RBM26 by Western Blot of Immunprecipitate.
    ab74197, at 1µg/ml, staining RBM26 in HeLa whole cell lysate immunoprecipitated using ab74197 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 1 second.

References

ab74197 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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