Product nameAnti-RBM26 antibody
DescriptionRabbit polyclonal to RBM26
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to a region between residues 450 and 500 of human RBM26 (NP_071401.3)
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab74198 was affinity purified using an epitope specific to RBM26 immobilized on solid support.
Our Abpromise guarantee covers the use of ab74198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 114 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceRBM26 (RNA binding motif protein 26) bears two RNA recognition motif (RRM) domains and a C3H1-type zinc finger. The function remains unknown.
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All lanes : Anti-RBM26 antibody (ab74198) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
Detection of RBM26 by Western Blot of Immunprecipitate.
ab74198, at 1µg/ml, staining RBM26 in HeLa whole cell lysate immunoprecipitated using ab74198 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second.
ab74198 has not yet been referenced specifically in any publications.