Anti-RBM28 antibody (ab150800)
Key features and details
- Rabbit polyclonal to RBM28
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RBM28 antibody
See all RBM28 primary antibodies -
Description
Rabbit polyclonal to RBM28 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to amino acids 504-622 of Human RBM28
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Positive control
- Human urinary bladder tissue; RT-4, U-251 MG and Human liver lysate; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 85 kDa.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 85 kDa. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Nucleolar component of the spliceosomal ribonucleoprotein complexes. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Alopecia, neurologic defects, and endocrinopathy syndrome -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus, nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 55131 Human
- Omim: 612074 Human
- SwissProt: Q9NW13 Human
- Unigene: 274263 Human
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Alternative names
- 2810480G15Rik antibody
- FLJ10377 antibody
- RBM 28 antibody
see all
Images
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All lanes : Anti-RBM28 antibody (ab150800) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 85 kDa -
Immunohistochemical analysis of paraffin embedded Human urinary bladder tissue labeling RBM28 with ab150800 antibody at a dilution of 1/20.
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Immunofluorescent analysis of A431 cells labeling RBM28 with ab150800 antibody at a dilution 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab150800 has been referenced in 1 publication.
- Jan SZ et al. Unraveling transcriptome dynamics in human spermatogenesis. Development 144:3659-3673 (2017). PubMed: 28935708