Key features and details
- Rabbit polyclonal to RBM28
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RBM28 antibody
See all RBM28 primary antibodies
DescriptionRabbit polyclonal to RBM28
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human urinary bladder tissue; RT-4, U-251 MG and Human liver lysate; A431 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 85 kDa.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionNucleolar component of the spliceosomal ribonucleoprotein complexes.
Tissue specificityUbiquitously expressed.
Involvement in diseaseAlopecia, neurologic defects, and endocrinopathy syndrome
Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
Cellular localizationNucleus, nucleolus.
- Information by UniProt
- 2810480G15Rik antibody
- FLJ10377 antibody
- RBM 28 antibody
All lanes : Anti-RBM28 antibody (ab150800) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 85 kDa
Immunohistochemical analysis of paraffin embedded Human urinary bladder tissue labeling RBM28 with ab150800 antibody at a dilution of 1/20.
Immunofluorescent analysis of A431 cells labeling RBM28 with ab150800 antibody at a dilution 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
ab150800 has been referenced in 1 publication.
- Jan SZ et al. Unraveling transcriptome dynamics in human spermatogenesis. Development 144:3659-3673 (2017). PubMed: 28935708