Overview

  • Product name

  • Description

    Rabbit polyclonal to RBM28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 504-622 of Human RBM28

  • Positive control

    • Human urinary bladder tissue; RT-4, U-251 MG and Human liver lysate; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab150800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500. Predicted molecular weight: 85 kDa.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Nucleolar component of the spliceosomal ribonucleoprotein complexes.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Alopecia, neurologic defects, and endocrinopathy syndrome
  • Sequence similarities

    Contains 4 RRM (RNA recognition motif) domains.
  • Cellular localization

    Nucleus, nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810480G15Rik antibody
    • FLJ10377 antibody
    • RBM 28 antibody
    • RBM28 antibody
    • RBM28_HUMAN antibody
    • RNA binding motif protein 28 antibody
    • RNA binding protein 28 antibody
    • RNA-binding motif protein 28 antibody
    • RNA-binding protein 28 antibody
    see all

Images

  • All lanes : Anti-RBM28 antibody (ab150800) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Predicted band size: 85 kDa

  • Immunohistochemical analysis of paraffin embedded Human urinary bladder tissue labeling RBM28 with ab150800 antibody at a dilution of 1/20.
  • Immunofluorescent analysis of A431 cells labeling RBM28 with ab150800 antibody at a dilution 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

References

This product has been referenced in:

See 1 Publication for this product

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