Anti-RBM28 antibody (ab150822)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RBM28 antibody
See all RBM28 primary antibodies -
Description
Rabbit polyclonal to RBM28 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to internal sequence amino acids 373-488 of Human RBM28 (UniProt ID: Q9NW13).
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Positive control
- Human hippocampus tissue; RT 4 and U 251 MG cell lysates, Human tonsil tissue lysate
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General notes
ab150822 should be gently mixed before use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab150822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. Predicted molecular weight: 86 kDa. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Nucleolar component of the spliceosomal ribonucleoprotein complexes. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Alopecia, neurologic defects, and endocrinopathy syndrome -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus, nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 55131 Human
- Entrez Gene: 68272 Mouse
- Entrez Gene: 312182 Rat
- Omim: 612074 Human
- SwissProt: Q9NW13 Human
- SwissProt: Q8CGC6 Mouse
- Unigene: 274263 Human
- Unigene: 40802 Mouse
see all -
Alternative names
- 2810480G15Rik antibody
- FLJ10377 antibody
- RBM 28 antibody
see all
Images
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Western blot - Anti-RBM28 antibody (ab150822)Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM28 antibody (ab150822)Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labelling RBM28 with ab150822 at 1/50 dilution. -
Western blot - Anti-RBM28 antibody (ab150822)All lanes : Anti-RBM28 antibody (ab150822) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : IgG/HSA-depleted Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 86 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (ab150822)Immunofluorescent analysis of Human cell line U-2 OS, labelling RBM28 with ab150822 at 1/50 (green). Red staining marks microtubules labelled with an anti-tubulin antibody.
Protocols
Datasheets and documents
References
ab150822 has not yet been referenced specifically in any publications.
