Anti-RBM28 antibody (ab150822)
Key features and details
- Rabbit polyclonal to RBM28
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RBM28 antibody
See all RBM28 primary antibodies -
Description
Rabbit polyclonal to RBM28 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to internal sequence amino acids 373-488 of Human RBM28 (UniProt ID: Q9NW13).
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Positive control
- Human hippocampus tissue; RT 4 and U 251 MG cell lysates, Human tonsil tissue lysate
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General notes
ab150822 should be gently mixed before use.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab150822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 86 kDa. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Nucleolar component of the spliceosomal ribonucleoprotein complexes. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Alopecia, neurologic defects, and endocrinopathy syndrome -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus, nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 55131 Human
- Entrez Gene: 68272 Mouse
- Entrez Gene: 312182 Rat
- Omim: 612074 Human
- SwissProt: Q9NW13 Human
- SwissProt: Q8CGC6 Mouse
- Unigene: 274263 Human
- Unigene: 40802 Mouse
see all -
Alternative names
- 2810480G15Rik antibody
- FLJ10377 antibody
- RBM 28 antibody
see all
Images
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Western blot - Anti-RBM28 antibody (ab150822)Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM28 antibody (ab150822)Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labelling RBM28 with ab150822 at 1/50 dilution. -
Western blot - Anti-RBM28 antibody (ab150822)All lanes : Anti-RBM28 antibody (ab150822) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : IgG/HSA-depleted Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 86 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (ab150822)Immunofluorescent analysis of Human cell line U-2 OS, labelling RBM28 with ab150822 at 1/50 (green). Red staining marks microtubules labelled with an anti-tubulin antibody.
Protocols
Datasheets and documents
References (0)
ab150822 has not yet been referenced specifically in any publications.
