Key features and details
- Mouse monoclonal [CL0296] to RBM3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-RBM3 antibody [CL0296]
See all RBM3 primary antibodies
DescriptionMouse monoclonal [CL0296] to RBM3
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- RT-4 cell lysate; Human breast cancer, colorectal tumor and prostate tumor tissues. WB: U-241 cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab211356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 - 10 µg/ml.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 17 kDa.|
FunctionCold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphoryaltion of translation initiation factors and active polysome formation.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsArg-105 is dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationNucleus. Cytoplasm. Cell projection > dendrite. Localizes in mRNA granules in dentrites.
- Information by UniProt
- 2600016C11Rik antibody
- IS1 RNPL antibody
- MGC105811 antibody
All lanes : Anti-RBM3 antibody [CL0296] (ab211356)
Lane 1 : U-251 cells, transfected with control siRNA
Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
Lane 3 : U-251 cells, transfected with target specific siRNA probe #2
Predicted band size: 17 kDa
Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling RBM3 with ab211356 at 1/500 dilution.
Immunofluorescence staining of U-2 OS cells staining RBM3 useing ab211356, showing specific staining in the nucleoplasm in green. Microtubule are visualized in red. Antibody staining is shown in green.
Anti-RBM3 antibody [CL0296] (ab211356) at 1/500 dilution + RT-4 cell lysate
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded Human colorectal tumor tissue labeling RBM3 with ab211356 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human prostate tumor tissue labeling RBM3 with ab211356 at 1/500 dilution.
ab211356 has not yet been referenced specifically in any publications.