Overview

  • Product name
    Anti-RBM3 antibody [EPR6061(2)]
    See all RBM3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6061(2)] to RBM3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RBM3 aa 100-200 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab188553)

  • Positive control
    • HepG2 cells; HeLa, HepG2, Jurkat, NIH/3T3 and RAW 264.7 cell lysates.
  • General notes

     

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/20000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.Can be blocked with Human RBM3 peptide (ab188553).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphoryaltion of translation initiation factors and active polysome formation.
    • Sequence similarities
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications
      Arg-105 is dimethylated, probably to asymmetric dimethylarginine.
      Phosphorylated.
    • Cellular localization
      Nucleus. Cytoplasm. Cell projection > dendrite. Localizes in mRNA granules in dentrites.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2600016C11Rik antibody
      • IS1 RNPL antibody
      • MGC105811 antibody
      • MGC118410 antibody
      • OTTHUMP00000025800 antibody
      • OTTHUMP00000025802 antibody
      • OTTMUSP00000019634 antibody
      • OTTMUSP00000019635 antibody
      • OTTMUSP00000019636 antibody
      • Putative RNA binding protein 3 antibody
      • Putative RNA-binding protein 3 antibody
      • Rbm3 antibody
      • RBM3_HUMAN antibody
      • RNA binding motif (RNP1, RRM) protein 3 antibody
      • RNA binding motif protein 3 antibody
      • RNA-binding motif protein 3 antibody
      • RNA-binding protein 3 antibody
      • RNPL antibody
      • RP23-27I6.7 antibody
      see all

    Images

    • All lanes : Anti-RBM3 antibody [EPR6061(2)] (ab134946) at 1/1000 dilution

      Lane 1 : RAW 264.7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : NIH/3T3 cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa

    • Immunofluorescence staining of HepG2 cells with purified ab134946 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • ab134946 staining RBM3 in the human cell line HepG2 (human hepatocellular carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Lei BB  et al. Mild Hypothermia Prevents NO-Induced Cytotoxicity in Human Neuroblastoma Cells Via Induction of COX-2. J Mol Neurosci N/A:N/A (2018). Read more (PubMed: 30471009) »
    • Yang HJ  et al. RNA-binding protein RBM3 prevents NO-induced apoptosis in human neuroblastoma cells by modulating p38 signaling and miR-143. Sci Rep 7:41738 (2017). Read more (PubMed: 28134320) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-4 of 4 Abreviews

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK-293)
    Permeabilization
    Yes - 0.3% Triton X-100 in Blocking Buffer
    Specification
    HEK-293
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 22 2018

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Tibialis Anterior Muscle)
    Gel Running Conditions
    Reduced Denaturing (8)
    Loading amount
    20 µg
    Specification
    Tibialis Anterior Muscle
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Ali Al-Rewashdy

    Verified customer

    Submitted Nov 17 2017

    This product is known to not work in this application or species.
    Application
    Immunoprecipitation
    Sample
    Rat Cell lysate - whole cell (R28 Rat Retinal Cell Line)
    Total protein in input
    1000 µg
    Treatment
    37¹C and 32¹C
    Immuno-precipitation step
    Other - Magnetic Beads Protein A/G
    Specification
    R28 Rat Retinal Cell Line

    Abcam user community

    Verified customer

    Submitted Mar 03 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    14 µg
    Specification
    Lymphocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 26 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up