Key features and details
- Rabbit polyclonal to RBM33
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- hypothetical protein MGC20460 antibody
- proline rich 8 antibody
- Proline-rich protein 8 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121695 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-RBM33 antibody (ab121695) at 1/100 dilution
Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
All lanes : Anti-RBM33 antibody (ab121695) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 cells lysate
Lane 3 : U-251 MG cells lysate
Lane 4 : Human plasma lysate
Lane 5 : Liver lysate
Lane 6 : Tonsil lysate
Immunohistochemical staining of paraffin embedded Human rectum tissue, using 1/40 ab121695.
ab121695 has not yet been referenced specifically in any publications.