Key features and details
- Rabbit polyclonal to RBM34
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RBM34 antibody
See all RBM34 primary antibodies
DescriptionRabbit polyclonal to RBM34
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla
Synthetic peptide corresponding to a region between residues 370 and 430 of human RBM34 (CAI21936.1).
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86787 was affinity purified using an epitope specific to RBM34 immobilized on solid support.
Our Abpromise guarantee covers the use of ab86787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 49 kDa.|
|IP||Use at 10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the RRM RBM34 family.
Contains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- KIAA0117 antibody
- Rbm34 antibody
- RBM34_HUMAN antibody
All lanes : Anti-RBM34 antibody (ab86787) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling RBM34 with ab86787 at 1/1000 (1µg/ml). Detection: DAB.
Detection of RBM34 by Western Blot of Immunprecipitate.
ab86787 at 0.4 µg/ml staining RBM34 in HeLa whole cell lysate immunoprecipitated using ab86787 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab86787 has not yet been referenced specifically in any publications.