Key features and details
- Mouse monoclonal [23A7.C9] to RBM35A + RBM35B
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG1
Product nameAnti-RBM35A + RBM35B antibody [23A7.C9]
DescriptionMouse monoclonal [23A7.C9] to RBM35A + RBM35B
Specificityab106555 recognises both Mouse RBM35A and RBM35B.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Horse, Cow, Dog, Human, Pig, Chimpanzee, Opossum
Recombinant full length Mouse RBM35A with a proprietary tag (NP_918944.2).
- RBM35A or RBM35B transfected 293T cell extracts
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notesab106555 was purified from tissue culture supernatant by Protein A chromatography followed by extensive dialysis against the buffer stated. The antibody is sterile filtered.
Our Abpromise guarantee covers the use of ab106555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 76, 77 kDa.|
FunctionmRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Tissue specificityEpithelial cell-specific. Epithelial-specific expression in diverse tissues and organs with particularly notable levels of expression in skin and gastrointestinal epithelia.
Sequence similaritiesBelongs to the ESRP family.
Contains 3 RRM (RNA recognition motif) domains.
- Information by UniProt
- Epithelial splicing regulatory protein 1 antibody
- Epithelial splicing regulatory protein 2 antibody
- Esrp1 antibody
All lanes : Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555) at 1/1000 dilution
Lane 1 : GFP transfected 293T cell extract
Lane 2 : RBM35A transfected 293T cell extract
Lane 3 : RBM35B transfected 293T cell extract
Lysates/proteins at 5 µg per lane.
All lanes : anti-mouse ECL antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 76, 77 kDa
ab106555 has been referenced in 4 publications.
- Chen KJ et al. Bleomycin-enhanced alternative splicing of fibroblast growth factor receptor 2 induces epithelial to mesenchymal transition in lung fibrosis. Biosci Rep 38:N/A (2018). PubMed: 30049844
- Jing J et al. Dynamic changes of urinary proteins in a rat model of acute hypercoagulable state induced by tranexamic acid. J Cell Physiol N/A:N/A (2018). PubMed: 30536986
- Chen L et al. Up-regulated miR-133a orchestrates epithelial-mesenchymal transition of airway epithelial cells. Sci Rep 8:15543 (2018). PubMed: 30341388
- Warzecha CC et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J 29:3286-300 (2010). PubMed: 20711167