• Product name

  • Description

    Rabbit polyclonal to RBM4
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Pig, Cynomolgus monkey
  • Immunogen

    Recombinant fragment corresponding to Human RBM4 aa 121-205.


    Database link: Q9BWF3

  • Positive control

    • WB: Mouse brain tissue lysate. IHC-P: Human heart tissue.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab222898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17,20,21,41 kDa.
IHC-P 1/20 - 1/200.


  • Function

    RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of EIF2C2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.
  • Tissue specificity

    Expressed in the cerebellum. Expressed in neurons and glial cells, including layers II neurons in the frontal cortex and CA1 pyramidal neurons in the hippocampus. Expressed in heart, liver, pancreas, skeletal muscle, placenta, primary fibroblasts and peripheral blood monocytes (at protein level). Ubiquitously expressed. Highly expressed in heart, placenta and skeletal muscle. Weakly expressed in pancreas, kidney, liver, lung and brain.
  • Sequence similarities

    Contains 1 CCHC-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.
  • Developmental stage

    Found to be expressed in fetal brain. Down-regulated in fetal Down syndrome (DS) brain.
  • Post-translational

    Phosphorylation on Ser-309 is induced upon cell muscle differentiation (By similarity). Phosphorylated. Phosphorylated in vitro on Ser-309 by SRPK1. Phosphorylation on Ser-309 is induced upon cell stress signaling, which alters its subcellular localization and may modulate its activity on IRES-mediated mRNA translation.
  • Cellular localization

    Nucleus. Nucleus > nucleolus. Nucleus speckle. Cytoplasm. Cytoplasmic granule. Undergoes continuous nucleocytoplasmic shuttling. Upon nuclear import colocalizes with SR proteins in nuclear speckles. Arsenite stress-induced phosphorylation increases its subcellular relocalization from the nucleus to the cytoplasm and to cytoplasmic stress granules (SG) via a p38 MAPK signaling pathway. Primarily localized in nucleus and nucleoli under cell growth conditions and accumulated in the cytoplasm and cytoplasm perinuclear granules upon muscle cell differentiation.
  • Information by UniProt
  • Database links

  • Alternative names

    • dkfzp547k0918 antibody
    • hLark antibody
    • lark antibody
    • Lark homolog antibody
    • lark homologue antibody
    • mgc75138 antibody
    • RBM4 antibody
    • rbm4 lark antibody
    • RBM4_HUMAN antibody
    • RNA binding motif 4 antibody
    • RNA binding motif 4a antibody
    • RNA-binding motif protein 4 antibody
    • RNA-binding motif protein 4a antibody
    • RNA-binding protein 4 antibody
    see all


  • Paraffin-embedded human heart tissue stained for RBM4 using ab222898 at 1/100 dilution in immunohistochemical analysis.

  • Anti-RBM4 antibody (ab222898) at 1/1000 dilution + Mouse brain tissue lysate

    Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 17,20,21,41 kDa
    Observed band size: 41 kDa
    why is the actual band size different from the predicted?


ab222898 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222898.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up