Product nameAnti-RBM42 antibody
DescriptionRabbit polyclonal to RBM42
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Synthetic peptide within Human RBM42 aa 100-150. The exact sequence is proprietary.
Database link: Q9BTD8
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: RBM42 IP in HEK-239T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000.|
RelevanceRNA binding motif protein 42 (RBM42) C-terminal domain interacts with HNRNPK C-terminla domain and RNA. RBM42 and HNRNPK are involved in the maintenance of cellular ATP levels during stress, possibly by protecting critical mRNAs. Upon stress response, RBM42 localizes with HNRNPK in cytoplasmic aggregates of stalled translational pre-initiation complexes called stress granules. There are 4 isoforms produced by alternative splicing.
Cellular localizationCytoplasmic and Nuclear
- MGC10433 antibody
- RNA binding motif protein 42 antibody
- RNA binding protein 42 antibody
All lanes : Anti-RBM42 antibody (ab241504) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
RBM42 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241504 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab241504 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 3 minutes.
ab241504 has not yet been referenced specifically in any publications.