Product nameAnti-RBM5 antibody
See all RBM5 primary antibodies
DescriptionRabbit polyclonal to RBM5
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from an internal region of Human RBM5
- Brain tissue; extracts from HUVEC cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionComponent of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.
Tissue specificityIsoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukemic cell lines.
Sequence similaritiesBelongs to the RBM5/RBM10 family.
Contains 1 C2H2-type zinc finger.
Contains 1 G-patch domain.
Contains 1 RanBP2-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains.
- Information by UniProt
- FLJ39876 antibody
- G15 antibody
- H37 antibody
Left: human brain tissue stained with ab69770 at 1/50 dilution; Right: the antibody was preincubated with the immunizing peptide.
All lanes : Anti-RBM5 antibody (ab69770) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with immunizing peptide at 10 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.