Recombinant Anti-RBM6 antibody [EPR15842] - C-terminal (ab188318)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15842] to RBM6 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RBM6 antibody [EPR15842] - C-terminal
See all RBM6 primary antibodies -
Description
Rabbit monoclonal [EPR15842] to RBM6 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Huvec, A549, HepG2, HeLa whole cell lysate (ab150035), A549 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15842 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 129 kDa (predicted molecular weight: 129 kDa).
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ICC/IF |
1/100.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 129 kDa (predicted molecular weight: 129 kDa). |
ICC/IF
1/100. |
Target
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Function
Specifically binds poly(G) RNA homopolymers in vitro. -
Tissue specificity
Ubiquitous in adults. -
Sequence similarities
Contains 1 G-patch domain.
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10180 Human
- Entrez Gene: 19654 Mouse
- Omim: 606886 Human
- SwissProt: P78332 Human
- Unigene: 696735 Human
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Alternative names
- 3G2 antibody
- 4930506F14Rik antibody
- DEF 3 antibody
see all
Images
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All lanes : Anti-RBM6 antibody [EPR15842] - C-terminal (ab188318) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 129 kDa -
All lanes : Anti-RBM6 antibody [EPR15842] - C-terminal (ab188318) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 129 kDa -
Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling RBM6 with ab188318at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Dylight® 488) at a 1/200 dilution. Counterstained with Dapi blue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab188318 has not yet been referenced specifically in any publications.