Recombinant
RabMAb

Anti-RBMX2 antibody [EPR14394] (ab180940)

Overview

  • Product name
    Anti-RBMX2 antibody [EPR14394]
  • Description
    Rabbit Monoclonal [EPR14394] to RBMX2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RBMX2 aa 150-300. The exact sequence is proprietary.
    Database link: Q9Y388

  • Positive control
    • HepG2, Hela and HT-1376; Human ovarian carcinoma and mouse testis tissues; HepG2 and Hela cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14394
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/100.
ICC/IF 1/100.

Target

Images

  • All lanes : Anti-RBMX2 antibody [EPR14394] (ab180940) at 1/10000 dilution

    Lane 1 : HepG2
    Lane 2 : HeLa

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Anti-RBMX2 antibody [EPR14394] (ab180940) at 1/2000 dilution + HT-1376 at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling RBMX2 with ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling RBMX2 with ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

  • Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining RBMX2 using ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).

  • Immunofluorescent analysis of -20℃ acetone-fixed HeLa cell line staining RBMX2 using ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).

References

ab180940 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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