Overview

  • Product name

    Anti-RBMY1A1 antibody [R12508(2)]
    See all RBMY1A1 primary antibodies
  • Description

    Rabbit monoclonal [R12508(2)] to RBMY1A1
  • Host species

    Rabbit
  • Specificity

    Based on sequence analysis, ab200202 recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RBMY1A1 aa 100-200. The exact sequence is proprietary.
    Database link: P0DJD3

  • Positive control

    • WB: Human testis lysate. IHC-P: Human testis tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab200202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 51, 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Function

    RNA-binding protein involved in pre-mRNA splicing. Required for sperm development. Acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN. Binds non-specifically to mRNAs.
  • Tissue specificity

    Testis-specific.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Developmental stage

    Expressed in all of the transcriptionally active stages of germ cell development from spermatogonia through spermatocytes to round spermatids.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • family 1 member A1 antibody
    • hRBMY antibody
    • MGC181956 antibody
    • RBM antibody
    • RBM1 antibody
    • RBM2 antibody
    • RBMY antibody
    • Rbmy1a1 antibody
    • RBMY1C antibody
    • RBY1A_HUMAN antibody
    • RNA binding motif protein 1 antibody
    • RNA binding motif protein 2 antibody
    • RNA binding motif protein Y chromosome family 1 member A1 antibody
    • RNA binding motif protein Y chromosome family 1 member A1/C antibody
    • RNA binding motif protein Y chromosome family 1 member antibody
    • RNA binding motif protein Y linked family 1 member A1 antibody
    • RNA-binding motif protein 1 antibody
    • RNA-binding motif protein 2 antibody
    • RNA-binding motif protein antibody
    • Y chromosome antibody
    • Y chromosome RNA recognition motif 1 antibody
    • YRRM1 antibody
    • YRRM2 antibody
    see all

Images

  • All lanes : Anti-RBMY1A1 antibody [R12508(2)] (ab200202) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate
    Lane 5 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 51,56 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Please note lanes 2-5 represent RBMY1A1 negative lysates.

    Based on sequence analysis, ab200202 recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RBMY1A1 with ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining of Human testis tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue with ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin. 

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Please note Human liver tissue represents a RBMY1A1 negative control.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab200202 has not yet been referenced specifically in any publications.

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