• Product name

    Anti-RBP-2 antibody
  • Description

    Rabbit polyclonal to RBP-2
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Ferret, Non human primates, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human RBP-2 aa 344-394.
    Database link: NP_115553.2

  • Positive control

    • 293T, HeLa, Jurkat and NIH3T3 whole cell lysates
  • General notes

     This product was previously labelled as PHAX



  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype



Our Abpromise guarantee covers the use of ab157096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
IP Use at 2-10 µg/mg of lysate.


  • Function

    A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.
  • Sequence similarities

    Belongs to the PHAX family.
  • Post-translational

    Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > Cajal body. Cytoplasm. Located in the nucleoplasm and Cajal bodies. Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ13193 antibody
    • PHAX antibody
    • PHAX_HUMAN antibody
    • Phosphorylated adapter RNA export protein antibody
    • Phosphorylated adaptor for RNA export antibody
    • RNA U small nuclear RNA export adapter protein antibody
    • RNA U, small nuclear RNA export adapter (phosphorylation regulated) antibody
    • RNUXA antibody
    see all


  • All lanes : Anti-RBP-2 antibody (ab157096) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa

    Exposure time: 10 seconds
  • All lanes : Anti-RBP-2 antibody (ab157096) at 0.4 µg/ml

    Lane 1 : NIH3T3 whole cell lysate at 50 µg
    Lane 2 : NIH3T3 whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa

    Exposure time: 3 minutes
  • Immunoprecipitation. ab157096 at 0.1 µg/ml staining by Western blot RBP-2 in 293T whole cell lysate immunoprecipitated using ab157096 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: control IgG.
    Predicted band size : 44 kDa.
    Exposure time: 10 seconds


This product has been referenced in:

  • Sheng P  et al. Dicer cleaves 5'-extended microRNA precursors originating from RNA polymerase II transcription start sites. Nucleic Acids Res 46:5737-5752 (2018). Read more (PubMed: 29746670) »
  • Xie M  et al. Mammalian 5'-capped microRNA precursors that generate a single microRNA. Cell 155:1568-80 (2013). Read more (PubMed: 24360278) »
See all 2 Publications for this product

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