Anti-RBP1 antibody (ab233296)
Key features and details
- Rabbit polyclonal to RBP1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-RBP1 antibody
See all RBP1 primary antibodies -
Description
Rabbit polyclonal to RBP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human RBP1 aa 1 to the C-terminus. Expressed in E. coli. N-terminal tags.
Database link: P09455-1 -
Positive control
- IHC-P: Human kidney tissue. WB: Recombinant human RBP1 protein. Pig liver tissue lysate. HEK-293T cell lysate. Rat testis tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Function
Intracellular transport of retinol. -
Tissue specificity
Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 537379 Cow
- Entrez Gene: 5947 Human
- Entrez Gene: 19659 Mouse
- Entrez Gene: 25056 Rat
- Omim: 180260 Human
- SwissProt: P02694 Cow
- SwissProt: P09455 Human
- SwissProt: Q00915 Mouse
see all -
Alternative names
- Cellular retinol binding protein antibody
- Cellular retinol-binding protein antibody
- Cellular retinol-binding protein I antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for RBP1 with ab233296 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-RBP1 antibody (ab233296) at 2 µg/ml
Lane 1 : Pig liver tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Rat testis tissue lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution -
Anti-RBP1 antibody (ab233296) at 2 µg/ml + Recombinant human RBP1 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233296 has not yet been referenced specifically in any publications.