Key features and details
- Rabbit polyclonal to RBP1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-RBP1 antibody
See all RBP1 primary antibodies
DescriptionRabbit polyclonal to RBP1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human, Pig
Predicted to work with: Mouse, Cow
Recombinant full length protein (His-tag) corresponding to Human RBP1 aa 2-135. Expressed in E. coli. N-terminal tags.
PVDFTGYWKMLVNENFEEYLRALDVNVALRKIANLLKPDKEIVQDGDHMI IRTLSTFRNYIMDFQVGKEFEEDLTGIDDRKCMTTVSWDGDKLQCVQKGE KEGRGWTQWIEGDELHLEMRVEGVVCKQVFKKVQ
Database link: P09455-1
- IHC-P: Human kidney tissue. WB: Recombinant human RBP1 protein. Pig liver tissue lysate. HEK-293T cell lysate. Rat testis tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
FunctionIntracellular transport of retinol.
Tissue specificityDetected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
- Information by UniProt
- Cellular retinol binding protein antibody
- Cellular retinol-binding protein antibody
- Cellular retinol-binding protein I antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for RBP1 with ab233296 at 10 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-RBP1 antibody (ab233296) at 2 µg/ml
Lane 1 : Pig liver tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Rat testis tissue lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Anti-RBP1 antibody (ab233296) at 2 µg/ml + Recombinant human RBP1 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233296 has not yet been referenced specifically in any publications.