Key features and details
- Goat polyclonal to RBP1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RBP1 antibody
See all RBP1 primary antibodies
DescriptionGoat polyclonal to RBP1
SpecificityAb31106 recognises RBP1.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
- NIH 3T3 lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAb31106 underwent ammonium sulphate precipitation prior to antigen affinity chromatography purification.
Our Abpromise guarantee covers the use of ab31106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
1 hour primary incubation is recommended for this product.
FunctionIntracellular transport of retinol.
Tissue specificityDetected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
- Information by UniProt
- Cellular retinol binding protein antibody
- Cellular retinol-binding protein antibody
- Cellular retinol-binding protein I antibody
Lane 1 : Anti-RBP1 antibody (ab31106) at 2 µg/ml
Lane 2 : Anti-RBP1 antibody (ab31106) at 0.5 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : U251 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 15 kDa
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr with ab31106 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Anti-RBP1 antibody (ab31106) at 0.1 µg/ml + NIH 3T3 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
ab31106 has been referenced in 1 publication.
- Zhu H et al. Proteomic analysis of testis biopsies in men treated with transient scrotal hyperthermia reveals the potential targets for contraceptive development. Proteomics 10:3480-93 (2010). WB ; Human . PubMed: 20815088