• Product name

  • Description

    Goat polyclonal to RBP1
  • Host species

  • Specificity

    Ab31106 recognises RBP1.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 121-134 of Human PRBPI

  • Positive control

    • NIH 3T3 lysate.



Our Abpromise guarantee covers the use of ab31106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).


  • Function

    Intracellular transport of retinol.
  • Tissue specificity

    Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.
  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Cellular retinol binding protein antibody
    • Cellular retinol-binding protein antibody
    • Cellular retinol-binding protein I antibody
    • CRABP I antibody
    • CRBP antibody
    • CRBP-I antibody
    • CRBP1 antibody
    • CRBPI antibody
    • RBP 1 antibody
    • RBP1 antibody
    • RBPC antibody
    • RET1_HUMAN antibody
    • Retinol binding protein 1 cellular antibody
    • Retinol-binding protein 1 antibody
    see all


  • Lane 1 : Anti-RBP1 antibody (ab31106) at 2 µg/ml
    Lane 2 : Anti-RBP1 antibody (ab31106) at 0.5 µg/ml

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : U251 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 15 kDa

  • Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr with ab31106 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

  • Anti-RBP1 antibody (ab31106) at 0.1 µg/ml + NIH 3T3 lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 1 hour


This product has been referenced in:

  • Zhu H  et al. Proteomic analysis of testis biopsies in men treated with transient scrotal hyperthermia reveals the potential targets for contraceptive development. Proteomics 10:3480-93 (2010). WB ; Human . Read more (PubMed: 20815088) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for your enquiry. NIH 3T3 lysates were prepared and western blotting done as below. I hope this information helps. Please do not hesitate to contact us if you need anything further. - Lysis. Cell pellets were washed with ice-cold PBS. 1 ml of RIPA buffer was added per 1E8 cells and incubated on ice for 20 min, vortexing 2-3 times, briefly. The lysate was aliquotted into 1.5 ml microfuge tubes and centrifuged at 13,000 rpm for 5 min in a microfuge. The supernatant was transferred into clean tubes and its protein concentration was measured with BioRad protein assay. The concentration was then adjusted to 5 mg/ml with RIPA lysis buffer. An equal volume of 2 x SDS sample buffer was then added and the cell lysate was boiled for 5 minutes. Lysates were stored at -80C until use.(RIPA buffer = 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml Aprotinin, 5 µg/ml Leupeptin, 1% Triton X-100, 1% Sodium deoxycholate, 0.1% SDS). - SDS PAGE. Samples were run at 200V constant on a 12% acrylamide SDS-PAGE mini gel - using Biorad Mini-Protean 3 kit and protocols. Before loading samples had 5% (v/v) 2-ME added and were boiled for 3 minutes. - Transfer. We used a Biorad Mini Trans-Blot, constant 100 V for 1 hour. Transfer Buffer was 20 mM Tris pH 8.0, 150 mM Glycine, 10% Methanol. We transferred to Millipore PVDF membrane and stained with Ponceau Red to evaluate the transfer. - Staining. The membrane was blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05% Tween-20) for 1 hr at room temperature with agitation. Primary antibody was incubated for 1 hr at room temperature with agitation. We used sigma secondary (Sigma anti-goat-HRP Product # A4174, use 1:3000) for 1 hr at room temperature with agitation. We washed with TBST three times after primary and secondary antibody, each wash lasting for 5-10 mins. ECL-plus (Amersham) was used rather than ECL, which is considerably more sensitive. Final detection was on autoradiography film.

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