Recombinant Anti-RBP2 antibody [EPR12334] (ab180494)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12334] to RBP2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RBP2 antibody [EPR12334]
See all RBP2 primary antibodies -
Description
Rabbit monoclonal [EPR12334] to RBP2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human small intestine and Caco2; Human small intestine tissue; SW480 cell line.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12334 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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Notes |
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ICC/IF
1/100. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
Target
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Function
Intracellular transport of retinol. -
Tissue specificity
Higher expression in adult small intestine and to a much lesser extent in fetal kidney. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5948 Human
- Entrez Gene: 19660 Mouse
- Entrez Gene: 407611 Pig
- Entrez Gene: 24710 Rat
- Omim: 180280 Human
- SwissProt: P50120 Human
- SwissProt: Q08652 Mouse
- SwissProt: P50121 Pig
see all -
Alternative names
- Cellular retinol binding protein II antibody
- Cellular retinol-binding protein II antibody
- CRABP II antibody
see all
Images
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Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Human small intestine tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 16 kDaBlocking buffer and concentration: 5% NFDM/TBST
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Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Caco-2 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Predicted band size: 16 kDaBlocking buffer and concentration: 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling RBP2 with ab180494 at a 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cell line staining RBP2 using ab180494 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/250. Counter stain: Dapi.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180494 has not yet been referenced specifically in any publications.