Recombinant
RabMAb

Recombinant Anti-RBP4 antibody [EPR18020-115] (ab188230)

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Overview

  • Product name

    Anti-RBP4 antibody [EPR18020-115]
    See all RBP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR18020-115] to RBP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse RBP4 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q00724

  • Positive control

    • WB: Mouse kidney tissue lysate and serum; Rat kidney tissue lysate; Hepa1-6 whole cell lysate. IP: Hepa1-6 whole cell lysate. IHC-P: Mouse and rat liver tissues. ICC/IF: Hepa1-6 cells. Flow Cyt: Hepa1-6 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

  • Involvement in disease

    Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium.

  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • OTTHUMP00000020114 antibody
      • OTTHUMP00000020115 antibody
      • OTTHUMP00000020116 antibody
      • Plasma retinol binding protein 4 antibody
      • Plasma retinol-binding protein antibody
      • Plasma retinol-binding protein(1-176) antibody
      • PRBP antibody
      • PRO2222 antibody
      • RBP antibody
      • RBP4 antibody
      • RDCCAS antibody
      • RET4_HUMAN antibody
      • Retinol binding protein 4 antibody
      • Retinol binding protein 4 interstitial antibody
      • Retinol binding protein 4 plasma antibody
      see all

    Images

    • Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      All lanes : Anti-RBP4 antibody [EPR18020-115] (ab188230) at 1/1000 dilution

      Lane 1 : Mouse serum
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
      Lane 4 : Rat kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 23 kDa
      Observed band size: 21 kDa
      why is the actual band size different from the predicted?



       

      Exposure times: Lanes 1, 2 and 4: 1 minute; Lane 3: 30 seconds.

      Blocking/dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of mouse liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of rat liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Immunofluorescent analysis of 4% paraformalehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling RBP4 with ab188230 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells. 

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow Cytometry - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Flow Cytometry - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling RBP4 with ab188230 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      RBP4 was immunoprecipitated from 0.35 mg of Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate with ab188230 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188230 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Hepa1-6 whole cell lysate 10 μg (Input).

      Lane 2: ab188230 IP in Hepa1-6 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188230 in Hepa1-6 whole cell lysate.

      Exposure time: 2 seconds.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    References

    ab188230 has not yet been referenced specifically in any publications.

    Publishing research using ab188230? Please let us know so that we can cite the reference in this datasheet.

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