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    rbp4-antibody-epr18020-115-ab188230.pdf

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Cardiovascular Blood Serum Proteins
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RecombinantRabMAb

Recombinant Anti-RBP4 antibody [EPR18020-115] (ab188230)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Flow Cytometry (Intracellular) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Anti-RBP4 antibody [EPR18020-115] (ab188230)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18020-115] to RBP4
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Recombinant Human RBP4 protein (ab63267)
Primary
Product image
Anti-Stra6 antibody (ab77616)

View more associated products

Overview

  • Product name

    Anti-RBP4 antibody [EPR18020-115]
    See all RBP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR18020-115] to RBP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse kidney tissue lysate and serum; Rat kidney tissue lysate; Hepa1-6 whole cell lysate. IP: Hepa1-6 whole cell lysate. IHC-P: Mouse and rat liver tissues. ICC/IF: Hepa1-6 cells. Flow Cyt (intra): Hepa1-6 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18020-115
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-RBP4 antibody [EPR18020-115] - BSA and Azide free (ab227487)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human RBP4 protein (ab63267)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/30.
Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/30.

Target

  • Function

    Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
  • Involvement in disease

    Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02753 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 19662 Mouse
    • Entrez Gene: 25703 Rat
    • SwissProt: Q00724 Mouse
    • SwissProt: P04916 Rat
    • Alternative names

      • OTTHUMP00000020114 antibody
      • OTTHUMP00000020115 antibody
      • OTTHUMP00000020116 antibody
      • Plasma retinol binding protein 4 antibody
      • Plasma retinol-binding protein antibody
      • Plasma retinol-binding protein(1-176) antibody
      • PRBP antibody
      • PRO2222 antibody
      • RBP antibody
      • RBP4 antibody
      • RDCCAS antibody
      • RET4_HUMAN antibody
      • Retinol binding protein 4 antibody
      • Retinol binding protein 4 interstitial antibody
      • Retinol binding protein 4 plasma antibody
      see all

    Images

    • Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      All lanes : Anti-RBP4 antibody [EPR18020-115] (ab188230) at 1/1000 dilution

      Lane 1 : Mouse serum
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
      Lane 4 : Rat kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 23 kDa
      Observed band size: 21 kDa why is the actual band size different from the predicted?



       

      Exposure times: Lanes 1, 2 and 4: 1 minute; Lane 3: 30 seconds.

      Blocking/dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of mouse liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of rat liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Immunofluorescent analysis of 4% paraformalehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling RBP4 with ab188230 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells. 

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Flow Cytometry (Intracellular) - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling RBP4 with ab188230 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      RBP4 was immunoprecipitated from 0.35 mg of Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate with ab188230 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188230 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Hepa1-6 whole cell lysate 10 μg (Input).

      Lane 2: ab188230 IP in Hepa1-6 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188230 in Hepa1-6 whole cell lysate.

      Exposure time: 2 seconds.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Anti-RBP4 antibody [EPR18020-115] (ab188230)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab188230? Please let us know so that we can cite the reference in this datasheet.

    ab188230 has been referenced in 2 publications.

    • Huang R  et al. Retinol-Binding Protein 4 Activates STRA6, Provoking Pancreatic ß-Cell Dysfunction in Type 2 Diabetes. Diabetes 70:449-463 (2021). PubMed: 33199363
    • Wang HM  et al. Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model. Nutr Metab (Lond) 17:5 (2020). PubMed: 31956331

    Customer reviews and Q&As

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