RecombinantRabMAb

Recombinant Anti-RBP4 antibody [EPR18020-115] (ab188230)

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Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Flow Cytometry - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)
  • Anti-RBP4 antibody [EPR18020-115] (ab188230)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18020-115] to RBP4
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-RBP4 antibody [EPR18020-115]
    See all RBP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR18020-115] to RBP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse RBP4 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q00724

  • Positive control

    • WB: Mouse kidney tissue lysate and serum; Rat kidney tissue lysate; Hepa1-6 whole cell lysate. IP: Hepa1-6 whole cell lysate. IHC-P: Mouse and rat liver tissues. ICC/IF: Hepa1-6 cells. Flow Cyt: Hepa1-6 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab188230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

  • Involvement in disease

    Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium.

  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • OTTHUMP00000020114 antibody
      • OTTHUMP00000020115 antibody
      • OTTHUMP00000020116 antibody
      • Plasma retinol binding protein 4 antibody
      • Plasma retinol-binding protein antibody
      • Plasma retinol-binding protein(1-176) antibody
      • PRBP antibody
      • PRO2222 antibody
      • RBP antibody
      • RBP4 antibody
      • RDCCAS antibody
      • RET4_HUMAN antibody
      • Retinol binding protein 4 antibody
      • Retinol binding protein 4 interstitial antibody
      • Retinol binding protein 4 plasma antibody
      see all

    Images

    • Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      All lanes : Anti-RBP4 antibody [EPR18020-115] (ab188230) at 1/1000 dilution

      Lane 1 : Mouse serum
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
      Lane 4 : Rat kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 23 kDa
      Observed band size: 21 kDa
      why is the actual band size different from the predicted?



       

      Exposure times: Lanes 1, 2 and 4: 1 minute; Lane 3: 30 seconds.

      Blocking/dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of mouse liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of rat liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Immunofluorescent analysis of 4% paraformalehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling RBP4 with ab188230 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells. 

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow Cytometry - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Flow Cytometry - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling RBP4 with ab188230 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)

      RBP4 was immunoprecipitated from 0.35 mg of Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate with ab188230 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188230 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Hepa1-6 whole cell lysate 10 μg (Input).

      Lane 2: ab188230 IP in Hepa1-6 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188230 in Hepa1-6 whole cell lysate.

      Exposure time: 2 seconds.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-RBP4 antibody [EPR18020-115] (ab188230)
      Anti-RBP4 antibody [EPR18020-115] (ab188230)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab188230? Please let us know so that we can cite the reference in this datasheet.

    ab188230 has been referenced in 1 publication.

    • Wang HM  et al. Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model. Nutr Metab (Lond) 17:5 (2020). PubMed: 31956331

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