Recombinant Anti-RBP4 antibody [EPR18020-115] (ab188230)
![Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)](https://www.abcam.com/ps/products/188/ab188230/Images/ab188230-289846-anti-rbp4-antibody-epr18020-115-western-blot.jpg "Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18020-115] to RBP4
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-RBP4 antibody [EPR18020-115]
See all RBP4 primary antibodies -
Description
Rabbit monoclonal [EPR18020-115] to RBP4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse kidney tissue lysate and serum; Rat kidney tissue lysate; Hepa1-6 whole cell lysate. IP: Hepa1-6 whole cell lysate. IHC-P: Mouse and rat liver tissues. ICC/IF: Hepa1-6 cells. Flow Cyt (intra): Hepa1-6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18020-115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
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| WB |
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
|
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| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/500.
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| IP |
1/30.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa). |
|
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/500. |
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IP
1/30. |
Target
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Function
Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. -
Involvement in disease
Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 19662 Mouse
- Entrez Gene: 25703 Rat
- SwissProt: Q00724 Mouse
- SwissProt: P04916 Rat
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Alternative names
- OTTHUMP00000020114 antibody
- OTTHUMP00000020115 antibody
- OTTHUMP00000020116 antibody
see all
Images
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Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)All lanes : Anti-RBP4 antibody [EPR18020-115] (ab188230) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lane 4 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1, 2 and 4: 1 minute; Lane 3: 30 seconds.
Blocking/dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)](https://www.abcam.com/ps/products/188/ab188230/Images/ab188230-289847-anti-rbp4-antibody-epr18020-115-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of mouse liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)](https://www.abcam.com/ps/products/188/ab188230/Images/ab188230-289848-anti-rbp4-antibody-epr18020-115-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of rat liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)](https://www.abcam.com/ps/products/188/ab188230/Images/ab188230-289851-anti-rbp4-antibody-epr18020-115-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)Immunofluorescent analysis of 4% paraformalehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling RBP4 with ab188230 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-RBP4 antibody [EPR18020-115] (ab188230)](https://www.abcam.com/ps/products/188/ab188230/Images/ab188230-289849-anti-rbp4-antibody-epr18020-115-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-RBP4 antibody [EPR18020-115] (ab188230)Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling RBP4 with ab188230 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)](https://www.abcam.com/ps/products/188/ab188230/Images/ab188230-289850-anti-rbp4-antibody-epr18020-115-immunoprecipitation.jpg)
Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)RBP4 was immunoprecipitated from 0.35 mg of Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate with ab188230 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188230 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Hepa1-6 whole cell lysate 10 μg (Input).
Lane 2: ab188230 IP in Hepa1-6 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188230 in Hepa1-6 whole cell lysate.
Exposure time: 2 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-RBP4 antibody [EPR18020-115] (ab188230)](https://www.abcam.com/ps/products/188/ab188230/Images/ab188230-7-benefits-of-recombinant-antibodies.png)
Anti-RBP4 antibody [EPR18020-115] (ab188230)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab188230 has been referenced in 2 publications.
- Huang R et al. Retinol-Binding Protein 4 Activates STRA6, Provoking Pancreatic ß-Cell Dysfunction in Type 2 Diabetes. Diabetes 70:449-463 (2021). PubMed: 33199363
- Wang HM et al. Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model. Nutr Metab (Lond) 17:5 (2020). PubMed: 31956331
