Product nameAnti-RBP4 antibody [EPR5879]
See all RBP4 primary antibodies
DescriptionRabbit monoclonal [EPR5879] to RBP4
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human RBP4 (UniProt ID: P02753).
- Human plasma, Human fetal liver, HepG2, and Human fetal kidney lysates, Human kidney and Human liver tissues
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 9.20 x 10 -13 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionDelivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
Involvement in diseaseDefects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium.
Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
- Information by UniProt
- OTTHUMP00000020114 antibody
- OTTHUMP00000020115 antibody
- OTTHUMP00000020116 antibody
All lanes : Anti-RBP4 antibody [EPR5879] (ab133559) at 1/10000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human fetal liver lysate
Lane 3 : HepG2 lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling RBP4 with ab133559 antibody at a dilution of 1/500.
Immunohistochemical analysis of paraffin embedded Human liver tissue labelling RBP4 with ab133559 antibody at a dilution of 1/500.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133559 has not yet been referenced specifically in any publications.