• Product name
    Anti-RBPJK antibody - ChIP Grade
    See all RBPJK primary antibodies
  • Description
    Rabbit polyclonal to RBPJK - ChIP Grade
  • Host species
  • Tested applications
    Suitable for: IP, ChIP, EMSA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human RBPJK.

    (Peptides available as ab26893)

  • Positive control
    • This antibody gave a positive signal in Human Testis and Mouse Testis tissue lysates and HeLa whole cell lysate.



Our Abpromise guarantee covers the use of ab25949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 20661424
EMSA Use at an assay dependent concentration. PubMed: 21991380
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ICC/IF Use at an assay dependent concentration.


  • Function
    Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
  • Sequence similarities
    Belongs to the Su(H) family.
    Contains 1 IPT/TIG domain.
  • Cellular localization
    Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI843960 antibody
    • AOS3 antibody
    • CBF 1 antibody
    • CBF-1 antibody
    • CBF1 antibody
    • csl antibody
    • IGKJRB antibody
    • IGKJRB1 antibody
    • J kappa recombination signal binding protein antibody
    • J kappa-recombination signal-binding protein antibody
    • KBF2 antibody
    • NY REN 30 antigen antibody
    • RBP J antibody
    • RBP J kappa antibody
    • RBP JK antibody
    • RBP-J antibody
    • RBP-J kappa antibody
    • RBP-JK antibody
    • Rbpj antibody
    • RBPJK antibody
    • RBPSUH antibody
    • recombination signal binding protein for immunoglobulin kappa J region antibody
    • Recombining binding protein suppressor of hairless antibody
    • Renal carcinoma antigen NY-REN-30 antibody
    • SUH antibody
    • SUH_HUMAN antibody
    see all


  • All lanes : ab25949 at 1 µg/ml

    Lane 1 : Testis (Human) Tissue Lysate at 20 µg
    Lane 2 : Testis (Mouse) Tissue Lysate at 20 µg
    Lane 3 : HeLa (Human) Whole cell lysate at 10 µg

    All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)

    Exposure time: 20 minutes

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Anti-RBPJK antibody - ChIP Grade (ab25949) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 20 µg

    Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 16 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25949 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody conjugated to HRP (ab191866), and visualised using ECL development solution ab133406.

  • RBPJK was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to RBPJK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab25949.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 56kDa, non specific bands -50kDa: This extra band is heavy chain contamination; RBPJK

  • All lanes : Anti-RBPJK antibody - ChIP Grade (ab25949) at 1/500 dilution

    Lane 1 : Mouse myoblast C2C12 whole cell lysate at 50 µg
    Lane 2 : Mouse myoblast C2C12 whole cell lysate at 75 µg

    All lanes : HRP-conjugated Goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

    Exposure time: 1 minute

    See Abreview


This product has been referenced in:
  • Szymula A  et al. Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment of transcription factors to the viral genome. PLoS Pathog 14:e1006890 (2018). Read more (PubMed: 29462212) »
  • Mouillesseaux KP  et al. Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6. Nat Commun 7:13247 (2016). ChIP . Read more (PubMed: 27834400) »

See all 12 Publications for this product

Customer reviews and Q&As

Thank you for taking the time to confirm this and sending us the qPCR results. The details you have kindly provided will provide us with vital information for our monitoring of product quality. It is regrettable the results have not been succes...

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Rat Cell lysate - nuclear (Cardiac fibroblasts)
Negative control
ChIP with rabbit IgG
Cardiac fibroblasts
Detection step
Real-time PCR
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
I didn't use a positive control

Mrs. Tal Golan Lagziel

Verified customer

Submitted Oct 31 2017

Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Mouse Cell lysate - whole cell (Mouse myoblast C2C12 cell line)
Mouse myoblast C2C12 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C


Verified customer

Submitted Oct 29 2013

I am very pleased to hear you would like to accept our offer and test ab25949 in mouse. Please order the antibody online if you prefer, and have the item deilvered to your Institute. This code will give you 1 free primary antibody before the expiration...

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Thank you very much for your interest in ab25949

To our knowledge, ab29549 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab25949 now, test it in mouse and submit feedback to us in the form o...

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Thank you for confirming this information and for your help and cooperation with this case. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As usual ...

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Thank you for taking the time to contact us. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this Anti-RBPJK antibody. I appreciate the time spent in the laboratory and understand the customer's concerns. It i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Human Cell (Human umbilical vein endothelial cells)
Human umbilical vein endothelial cells
Yes - 1% Triton X-100

Abcam user community

Verified customer

Submitted Apr 14 2010


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