Anti-RBPMS antibody (ab152101)
Key features and details
- Rabbit polyclonal to RBPMS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RBPMS antibody
See all RBPMS primary antibodies -
Description
Rabbit polyclonal to RBPMS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 28-202 of Human RBPMS (Q93062; NP_001008712).
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Positive control
- Wild-type HeLa, A549 and Mouse heart whole cell lysates. IHC-P: Human lung adenocarcinoma tissue. ICC: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
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IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA. -
Tissue specificity
Ubiquitously expressed, at various levels depending on the isoform and the tissue. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The RRM domain is necessary for interaction with SMAD4. Both the RRM domain and the C-terminus are required for TGFB1/Smad-mediated transactivation activity. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 614417 Cow
- Entrez Gene: 11030 Human
- Entrez Gene: 19663 Mouse
- Omim: 601558 Human
- SwissProt: Q93062 Human
- SwissProt: Q9WVB0 Mouse
- Unigene: 334587 Human
- Unigene: 323997 Mouse
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Alternative names
- FLJ32971 antibody
- Heart and RRM expressed sequence antibody
- Hermes antibody
see all
Images
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All lanes : Anti-RBPMS antibody (ab152101) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RBPMS Knockout HeLa cell lysate
Lane 3 : RT-4 cell lysate
Lane 4 : U-251 MG cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab152101 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab152101 was shown to react with RBPMS in wild-type HeLa cells in western blot. The bands observed in RBPMS knockout cell line ab264697 (RBPMS knockout cell lysate ab258631) below 30 kDa may represent truncated forms and cleaved fragments. This has not been investigated further and the functional properties of the gene product have not been determined. HeLa wild-type and RBPMS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab152101 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RBPMS with ab152101 at 1/250 dilution.
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Anti-RBPMS antibody (ab152101) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 24 kDa
12% SDS PAGE -
Anti-RBPMS antibody (ab152101) at 1/1000 dilution + Mouse heart whole cell lysate at 50 µg
Predicted band size: 24 kDa
12% SDS PAGE -
Immunofluorescent analysis of methanol-fixed A549 cells labeling RBPMS with ab152101 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab152101 has been referenced in 11 publications.
- D'Souza SP et al. Retinal patterns and the cellular repertoire of neuropsin (Opn5) retinal ganglion cells. J Comp Neurol 530:1247-1262 (2022). PubMed: 34743323
- Zhang Y et al. Sirt5-mediated desuccinylation of OPTN protects retinal ganglion cells from autophagic flux blockade in diabetic retinopathy. Cell Death Discov 8:63 (2022). PubMed: 35165261
- Jin Z et al. Identification of the Transcription Co-Factor-Related Gene Signature and Risk Score Model for Osteosarcoma. Front Genet 13:862803 (2022). PubMed: 35734428
- Rowe AA et al. Long-term progression of retinal degeneration in a preclinical model of CLN7 Batten disease as a baseline for testing clinical therapeutics. EBioMedicine 85:104314 (2022). PubMed: 36374771
- Mou Q et al. Modulation of Sirt1-mTORC1 Pathway in Microglia Attenuates Retinal Ganglion Cell Loss After Optic Nerve Injury. J Inflamm Res 14:6857-6869 (2021). PubMed: 34934336