Overview

  • Product name

  • Description

    Rabbit polyclonal to RBPMS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 28-202 of Human RBPMS (Q93062; NP_001008712).

  • Positive control

    • A549 and Mouse heart whole cell lysates; A549 cells; Human lung adenocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab152101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.
  • Tissue specificity

    Ubiquitously expressed, at various levels depending on the isoform and the tissue.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Domain

    The RRM domain is necessary for interaction with SMAD4. Both the RRM domain and the C-terminus are required for TGFB1/Smad-mediated transactivation activity.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ32971 antibody
    • Heart and RRM expressed sequence antibody
    • Hermes antibody
    • RBP MS antibody
    • RBP-MS antibody
    • Rbpms antibody
    • RBPMS_HUMAN antibody
    • RNA binding protein gene with multiple splicing antibody
    • RNA-binding protein with multiple splicing antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RBPMS with ab152101 at 1/250 dilution.
  • Anti-RBPMS antibody (ab152101) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 24 kDa



    12% SDS PAGE
  • Anti-RBPMS antibody (ab152101) at 1/1000 dilution + Mouse heart whole cell lysate at 50 µg

    Predicted band size: 24 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed A549 cells labeling RBPMS with ab152101 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Davis BM  et al. Topical Coenzyme Q10 demonstrates mitochondrial-mediated neuroprotection in a rodent model of ocular hypertension. Mitochondrion 36:114-123 (2017). ICC/IF . Read more (PubMed: 28549843) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab152101.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up