Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.
Tissue specificityUbiquitously expressed, at various levels depending on the isoform and the tissue.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
DomainThe RRM domain is necessary for interaction with SMAD4. Both the RRM domain and the C-terminus are required for TGFB1/Smad-mediated transactivation activity.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- FLJ32971 antibody
- Heart and RRM expressed sequence antibody
- Hermes antibody
All lanes : Anti-RBPMS antibody (ab67078) at 1/500 dilution
Lane 1 : RBPMS transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 21 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
ab67078 has not yet been referenced specifically in any publications.