Overview

  • Product name
    Anti-RBPSUHL antibody - N-terminal
  • Description
    Rabbit polyclonal to RBPSUHL - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RBPSUHL aa 15-44 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9UBG7

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab170720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 57 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    RBPSUHL is a protein similar in sequence to the mouse RPB-L protein and Drosophila suppressor of hairless protein. In mouse, recombining binding protein L (RBP-L) is a transcription factor that binds to DNA sequences almost identical to that bound by the Notch receptor signalling pathway transcription factor RBP-J. However, unlike RBP-J, RBP-L does not interact with Notch receptors. RBP-L has been shown to activate transcription in concert with Epstein-Barr virus nuclear antigen-2 (EBNA2).
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • hCG_38385 antibody
    • RBP L antibody
    • RBP-L antibody
    • RBPL antibody
    • RBPSUHL antibody
    • Recombination signal binding protein for immunoglobulin kappa J region like antibody
    • Recombining binding protein L antibody
    • Recombining binding protein suppressor of hairless (Drosophila)-like antibody
    • Recombining binding protein suppressor of hairless (Drosophila)-like, isoform CRA_b antibody
    • Recombining binding protein suppressor of hairless like protein antibody
    • RP3 453C12.2 002 antibody
    • SUH antibody
    • SUHL antibody
    • Transcription factor RBP L antibody
    • Transcription factor RBP-L antibody
    • Transcription factor RBPL antibody
    see all

Images

  • Anti-RBPSUHL antibody - N-terminal (ab170720) at 1/100 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size: 57 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling RBPSUHL with ab170720 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometric analysis of Jurkat cells labeling RBPSUHL with ab170720 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

References

ab170720 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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