Product nameAnti-RBX1 antibody [EPR6850(B)]
See all RBX1 primary antibodies
DescriptionRabbit monoclonal [EPR6850(B)] to RBX1
Tested applicationsSuitable for: Flow Cyt, IP, ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human RBX1 aa 50-150 (C terminal).
Database link: P62877
- Human colon tissue; MCF7, HepG2, HeLa and Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.18 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- HeLa nuclear extract lysate (ab14655)
- HepG2 cytoplasmic extract lysate (ab14659)
- HepG2 nuclear extract lysate (ab14660)
- Jurkat cytoplasmic extract lysate (ab14842)
- Jurkat nuclear extract lysate (ab14844)
- MCF7 nuclear extract lysate (ab14860)
- HeLa whole cell lysate (ab29545)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
Our Abpromise guarantee covers the use of ab133565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 12 kDa.|
FunctionE3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
Tissue specificityWidely expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RING-box family.
Contains 1 RING-type zinc finger.
DomainThe RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- BA554C12.1 antibody
- E3 ubiquitin-protein ligase RBX1 antibody
- FLJ60363 antibody
All lanes : Anti-RBX1 antibody [EPR6850(B)] (ab133565) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 12 kDa
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling RBX1 with ab133565 at 1/100 dilution.
Overlay histogram showing HeLa cells stained with ab133565 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133565, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab133565 at 1/70 immunoprecipitating RBX1 in HepG2 whole cell lysate.
For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Salehi-Tabar R et al. The Tumor Suppressor FBW7 and the Vitamin D Receptor Are Mutual Cofactors in Protein Turnover and Transcriptional Regulation. Mol Cancer Res 17:709-719 (2019). Read more (PubMed: 30606768) »
- Li Y et al. Heterozygous deletion of chromosome 17p renders prostate cancer vulnerable to inhibition of RNA polymerase II. Nat Commun 9:4394 (2018). Read more (PubMed: 30349055) »