Key features and details
- Rabbit polyclonal to RC3H1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RC3H1 antibody
See all RC3H1 primary antibodies
DescriptionRabbit polyclonal to RC3H1
SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to a region between residues 500 and 550 of human RC3H1
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70195 was affinity purified using an epitope specific to RC3H1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRepresses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1.
Tissue specificityWidely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.
Sequence similaritiesContains 1 C3H1-type zinc finger.
Contains 1 RING-type zinc finger.
Cellular localizationCytoplasm. Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA.
- Information by UniProt
- KIAA2025 antibody
- Rc3h1 antibody
- RC3H1_HUMAN antibody
All lanes : Anti-RC3H1 antibody (ab70195) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 126 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Western Blot showing ab70195, at 1µg/ml, staining RC3H1 in HeLa whole cell lysates immunoprecipitated using ab70195 at 3µg/ml (1 mg/IP; 1/4 of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 minutes.
ab70195 has not yet been referenced specifically in any publications.