Overview

  • Product name

  • Description

    Rabbit polyclonal to RC3H1
  • Host species

    Rabbit
  • Specificity

    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 500 and 550 of human RC3H1

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/500 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1.
    • Tissue specificity

      Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.
    • Sequence similarities

      Contains 1 C3H1-type zinc finger.
      Contains 1 RING-type zinc finger.
    • Cellular localization

      Cytoplasm. Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA.
    • Information by UniProt
    • Database links

    • Alternative names

      • KIAA2025 antibody
      • Rc3h1 antibody
      • RC3H1_HUMAN antibody
      • RGD1563512 antibody
      • RING finger and C3H zinc finger protein 1 antibody
      • Ring Finger and CCCH type Zinc Finger Domain 1 antibody
      • RING finger protein 198 antibody
      • RNF198 antibody
      • Roquin antibody
      • RP5 1198E17.5 antibody
      • Sanroque protein antibody
      see all

    Images

    • All lanes : Anti-RC3H1 antibody (ab70195) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 126 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes
    • Western Blot showing ab70195, at 1µg/ml, staining RC3H1 in HeLa whole cell lysates immunoprecipitated using ab70195 at 3µg/ml (1 mg/IP; 1/4 of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 minutes.

    References

    ab70195 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70195.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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