Key features and details
- Rabbit polyclonal to RC3H1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RC3H1 antibody
See all RC3H1 primary antibodies
DescriptionRabbit polyclonal to RC3H1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between C terminal residues 1075 and 1133 of human RC3H1 (NP_742068.1)
- HeLa and 293T whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70196 was affinity purified using an epitope specific to RC3H1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRepresses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1.
Tissue specificityWidely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.
Sequence similaritiesContains 1 C3H1-type zinc finger.
Contains 1 RING-type zinc finger.
Cellular localizationCytoplasm. Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA.
- Information by UniProt
- KIAA2025 antibody
- Rc3h1 antibody
- RC3H1_HUMAN antibody
All lanes : Anti-RC3H1 antibody (ab70196) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 126 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Western Blot showing Lane 2: ab70196 at 0.04µg/ml staining RC3H1 in HeLa whole cell lysates immunoprecipitated using ab70196 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 1: an irrelevant antibody was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab70196 has not yet been referenced specifically in any publications.