Overview

  • Product name

    Anti-RC3H1 antibody - C-terminal
    See all RC3H1 primary antibodies
  • Description

    Rabbit polyclonal to RC3H1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RC3H1 (C terminal). The exact sequence is proprietary.
    Database link: Q5TC82

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; RC3H1-transfected HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 126 kDa.

Target

  • Function

    Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1.
  • Tissue specificity

    Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.
  • Sequence similarities

    Contains 1 C3H1-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Cytoplasm. Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA2025 antibody
    • Rc3h1 antibody
    • RC3H1_HUMAN antibody
    • RGD1563512 antibody
    • RING finger and C3H zinc finger protein 1 antibody
    • Ring Finger and CCCH type Zinc Finger Domain 1 antibody
    • RING finger protein 198 antibody
    • RNF198 antibody
    • Roquin antibody
    • RP5 1198E17.5 antibody
    • Sanroque protein antibody
    see all

Images

  • All lanes : Anti-RC3H1 antibody - C-terminal (ab229545) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 126 kDa



    5% SDS-PAGE gel.

  • All lanes : Anti-RC3H1 antibody - C-terminal (ab229545) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : RC3H1 (including 3xFlag-tag) transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 126 kDa



    5% SDS-PAGE gel.

References

ab229545 has not yet been referenced specifically in any publications.

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