Overview

  • Product name

  • Description

    Rabbit polyclonal to RCAS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human RCAS1 aa 163-213. The exact sequence is proprietary. (NP_004206.1).
    Database link: O00559

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab225701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 24 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
  • Tissue specificity

    Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
  • Domain

    The coiled coil domain is necessary for the homodimerization.
  • Cellular localization

    Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAG9 antibody
    • Cancer associated surface antigen antibody
    • Cancer associated surface antigen RCAS1 antibody
    • Cancer-associated surface antigen RCAS1 antibody
    • EB9 antibody
    • EBAG 9 antibody
    • EBAG9 antibody
    • Estrogen receptor binding fragment associated gene 9 protein antibody
    • Estrogen receptor binding site associated antigen 9 antibody
    • estrogen receptor binding site associated, antigen, 9 antibody
    • Estrogen receptor-binding fragment-associated gene 9 protein antibody
    • PDAF antibody
    • RCAS 1 antibody
    • RCAS1 antibody
    • RCAS1_HUMAN antibody
    • Receptor binding cancer antigen expressed on SiSo cells antibody
    • Receptor-binding cancer antigen expressed on SiSo cells antibody
    see all

Images

  • All lanes : Anti-RCAS1 antibody (ab225701) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 24 kDa


    Exposure time: 30 seconds
  • RCAS1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225701 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225701 at 0.4 µg/ml.

    Lane 1: ab225701 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 seconds.

References

ab225701 has not yet been referenced specifically in any publications.

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