Recombinant Anti-RCAS1 antibody [EPR16568] (ab200348)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16568] to RCAS1
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-RCAS1 antibody [EPR16568]
See all RCAS1 primary antibodies -
Description
Rabbit monoclonal [EPR16568] to RCAS1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Jurkat, 293 and T-47D whole cell lysates; Human fetal brain and fetal kidney lysates. ICC/IF: HEK293 and T-47D cells. IP: HEK293 whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16568 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/2000.
|
|
IP |
1/80.
|
|
WB |
1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 24, 29 kDa).
|
Notes |
---|
ICC/IF
1/2000. |
IP
1/80. |
WB
1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 24, 29 kDa). |
Target
-
Function
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases. -
Tissue specificity
Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs. -
Domain
The coiled coil domain is necessary for the homodimerization. -
Cellular localization
Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain. - Information by UniProt
-
Database links
- Entrez Gene: 9166 Human
- Omim: 605772 Human
- SwissProt: O00559 Human
- Unigene: 409368 Human
-
Alternative names
- BAG9 antibody
- Cancer associated surface antigen antibody
- Cancer associated surface antigen RCAS1 antibody
see all
Images
-
All lanes : Anti-RCAS1 antibody [EPR16568] (ab200348) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood ) whole cell lysate
Lane 2 : 293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24, 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized T-47D (Human ductal breast epithelial tumor cell line) cells, labeling RCAS1 with ab200348 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on T47D cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab200348 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling RCAS1 with ab200348 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HEK293 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab200348 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
Anti-RCAS1 antibody [EPR16568] (ab200348) at 1/1000 dilution + T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24, 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-RCAS1 antibody [EPR16568] (ab200348) at 1/5000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 24, 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
RCAS1 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200348 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab200348 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK293 whole cell lysate 10 µg (Input). Lane 2: ab200348 IP in HEK293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200348 in HEK293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab200348 has not yet been referenced specifically in any publications.