Overview

  • Product name
    Anti-RCBTB2 antibody - N-terminal
    See all RCBTB2 primary antibodies
  • Description
    Rabbit polyclonal to RCBTB2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RCBTB2 aa 78-107 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O95199

  • Positive control
    • Mouse thymus tissue lysate; Human brain tissue; Jurkat cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab175505 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 60 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • CHC1 L antibody
    • CHC1L antibody
    • Chromosome condensation 1 like antibody
    • RCBT2 antibody
    • RCBT2_HUMAN antibody
    • Rcbtb2 antibody
    • RCC1 and BTB domain containing protein 2 antibody
    • RCC1 and BTB domain-containing protein 2 antibody
    • RCC1 like G exchanging factor antibody
    • RCC1 like G exchanging factor RLG antibody
    • Regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2 antibody
    • Regulator of chromosome condensation (RCC1) and BTB domain containing protein 2 antibody
    • Regulator of chromosome condensation and BTB domain containing protein 2 antibody
    • RLG antibody
    see all

Images

  • Anti-RCBTB2 antibody - N-terminal (ab175505) at 1/1000 dilution + Mouse thymus tissue lysate at 35 µg

    Predicted band size: 60 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human brain tissue labeling RCBTB2 with ab175505 at 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Flow cytometric analysis of Jurkat cells labeling RCBTB2 with ab175505 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175505 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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